Ackermann M R, Register K B, Gentry-Weeks C, Gwaltney S M, Magyar T
USDA/Agricultural Research Service/National Animal Disease Center, Ames, Iowa, USA.
J Comp Pathol. 1997 Jan;116(1):55-61. doi: 10.1016/s0021-9975(97)80043-6.
Studies of virulence factors of Bordetella bronchiseptica require a suitable system. Such a system was devised in colostrum-deprived, caesarean-derived pigs, aged 7 d. In two different experiments, pigs (n = 11) were inoculated intranasally with 10(6) colony-forming units of the virulent strain 4609. In the same way, further pigs (n = 11) were inoculated with a strain (B133) of unknown virulence. No significant differences between 4609 and B133 colonization were seen. However, colonization of the turbinates was significantly higher than that of the trachea, lung and tonsil, and a significantly higher degree of colonization was present at 11 d post-inoculation (PI) than at 15 days. Moderate turbinate atrophy was present by 11 d PI, and peribronchiolar fibrosis was present at 15 days. Immunocytochemical methods showed that all pigs had bacterial antigen in the ciliated cells of the turbinates and trachea, and in the lung; some pigs also had antigen in the bronchi. Bacterial antigen was present in some bronchioles and within the cytoplasm of pulmonary macrophages and neutrophils. This model should prove useful for comparing strains of B. bronchiseptica and isogenic mutants deficient in putative virulence factors.
支气管败血波氏杆菌毒力因子的研究需要一个合适的系统。这样一个系统是在7日龄初乳剥夺、剖腹产获得的仔猪中设计的。在两个不同的实验中，给11头仔猪经鼻接种10(6)个菌落形成单位的强毒株4609。同样，给另外11头仔猪接种一株毒力未知的菌株（B133）。在4609和B133的定植方面未观察到显著差异。然而，鼻甲的定植明显高于气管、肺和扁桃体，接种后11天（PI）的定植程度明显高于15天。接种后11天出现中度鼻甲萎缩，15天时出现细支气管周围纤维化。免疫细胞化学方法显示，所有仔猪的鼻甲、气管和肺的纤毛细胞中都有细菌抗原；一些仔猪的支气管中也有抗原。细菌抗原存在于一些细支气管以及肺巨噬细胞和中性粒细胞的细胞质中。该模型对于比较支气管败血波氏杆菌菌株和假定毒力因子缺陷的同基因突变体应该是有用的。
