从百日咳博德特氏菌中鉴定并克隆 waaF（rfaF），并用于构建具有深度粗糙型脂多糖的博德特氏菌属突变体。
Identification and cloning of waaF (rfaF) from Bordetella pertussis and use to generate mutants of Bordetella spp. with deep rough lipopolysaccharide.
作者信息
Allen A G, Isobe T, Maskell D J
机构信息
Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, United Kingdom.
出版信息
J Bacteriol. 1998 Jan;180(1):35-40. doi: 10.1128/JB.180.1.35-40.1998.
Abstract
A DNA locus from Bordetella pertussis capable of reconstituting lipopolysaccharide (LPS) O-antigen biosynthesis in Salmonella typhimurium SL3789 (rfaF511) has been isolated, by using selection with the antibiotic novobiocin. DNA within the locus encodes a protein with amino acid sequence similarity to heptosyltransferase II, encoded by waaF (previously rfaF) in other gram-negative bacteria. Mutation of this gene in B. pertussis, Bordetella parapertussis, and Bordetella bronchiseptica by allelic exchange generated bacteria with deep rough LPS phenotypes consistent with the proposed function of the gene as an inner core heptosyltransferase. These are the first LPS mutants generated in B. parapertussis and B. bronchiseptica and the first deep rough mutants of any of the bordetellae.
摘要
通过使用新霉素进行筛选，已从百日咳博德特氏菌中分离出一个能够在鼠伤寒沙门氏菌SL3789（rfaF511）中重建脂多糖（LPS）O抗原生物合成的DNA位点。该位点内的DNA编码一种蛋白质，其氨基酸序列与其他革兰氏阴性细菌中由waaF（以前称为rfaF）编码的庚糖基转移酶II相似。通过等位基因交换对百日咳博德特氏菌、副百日咳博德特氏菌和支气管败血博德特氏菌中的该基因进行突变，产生了具有深粗糙LPS表型的细菌，这与该基因作为内核庚糖基转移酶的推测功能一致。这些是在副百日咳博德特氏菌和支气管败血博德特氏菌中产生的首批LPS突变体，也是所有博德特氏菌中的首批深粗糙突变体。
相似文献
1
Identification and cloning of waaF (rfaF) from Bordetella pertussis and use to generate mutants of Bordetella spp. with deep rough lipopolysaccharide.
J Bacteriol. 1998 Jan;180(1):35-40. doi: 10.1128/JB.180.1.35-40.1998.
2
Molecular and functional analysis of the lipopolysaccharide biosynthesis locus wlb from Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica.
Mol Microbiol. 1998 Jul;29(1):27-38. doi: 10.1046/j.1365-2958.1998.00878.x.
3
The identification, cloning and mutagenesis of a genetic locus required for lipopolysaccharide biosynthesis in Bordetella pertussis.
Mol Microbiol. 1996 Jan;19(1):37-52. doi: 10.1046/j.1365-2958.1996.354877.x.
4
Molecular characterization of a gene region involved in lipopolysaccharide biosynthesis in Bradyrhizobium japonicum: cloning, sequencing and expression of rfaf gene.
FEMS Microbiol Lett. 2000 Sep 1;190(1):109-14. doi: 10.1111/j.1574-6968.2000.tb09271.x.
5
Representational difference analysis identifies a strain-specific LPS biosynthesis locus in Bordetella spp.
Mol Gen Genet. 1999 Aug;262(1):189-98. doi: 10.1007/s004380051074.
6
Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae.
Infect Immun. 1999 Aug;67(8):3763-7. doi: 10.1128/IAI.67.8.3763-3767.1999.
7
Structural and genetic analysis of the bvg locus in Bordetella species.
Mol Microbiol. 1991 Oct;5(10):2481-91. doi: 10.1111/j.1365-2958.1991.tb02093.x.
8
A role for lipopolysaccharide in turkey tracheal colonization by Bordetella avium as demonstrated in vivo and in vitro.
Mol Microbiol. 2000 Jun;36(6):1425-35. doi: 10.1046/j.1365-2958.2000.01963.x.
9
Identification of the ADP-L-glycero-D-manno-heptose-6-epimerase (rfaD) and heptosyltransferase II (rfaF) biosynthesis genes from nontypeable Haemophilus influenzae 2019.
Infect Immun. 1997 Apr;65(4):1377-86. doi: 10.1128/iai.65.4.1377-1386.1997.
10
Isolation and characterization of two genes, waaC (rfaC) and waaF (rfaF), involved in Pseudomonas aeruginosa serotype O5 inner-core biosynthesis.
J Bacteriol. 1997 Jun;179(11):3451-7. doi: 10.1128/jb.179.11.3451-3457.1997.
引用本文的文献
1
Construction of the waaF Subunit and DNA Vaccine Against in Cow Mastitis and Preliminary Study on Their Immunogenicity.
Front Vet Sci. 2022 May 12;9:877685. doi: 10.3389/fvets.2022.877685. eCollection 2022.
2
A Newly Discovered Drug Resistance Gene In .
Infect Drug Resist. 2019 Nov 12;12:3507-3514. doi: 10.2147/IDR.S231152. eCollection 2019.
3
Vaccine-Mediated Activation of Human TLR4 Is Affected by Modulation of Culture Conditions during Whole-Cell Pertussis Vaccine Preparation.
PLoS One. 2016 Aug 22;11(8):e0161428. doi: 10.1371/journal.pone.0161428. eCollection 2016.
4
Identification of a novel lipopolysaccharide core biosynthesis gene cluster in Bordetella pertussis, and influence of core structure and lipid A glucosamine substitution on endotoxic activity.
Infect Immun. 2009 Jul;77(7):2602-11. doi: 10.1128/IAI.00033-09. Epub 2009 Apr 13.
5
Bordetella pertussis, the causative agent of whooping cough, evolved from a distinct, human-associated lineage of B. bronchiseptica.
PLoS Pathog. 2005 Dec;1(4):e45. doi: 10.1371/journal.ppat.0010045. Epub 2005 Dec 30.
6
OpsX from Haemophilus influenzae represents a novel type of heptosyltransferase I in lipopolysaccharide biosynthesis.
J Bacteriol. 2005 Sep;187(17):6242-7. doi: 10.1128/JB.187.17.6242-6247.2005.
7
Interactions of pulmonary collectins with Bordetella bronchiseptica and Bordetella pertussis lipopolysaccharide elucidate the structural basis of their antimicrobial activities.
Infect Immun. 2004 Dec;72(12):7124-30. doi: 10.1128/IAI.72.12.7124-7130.2004.
8
Comparison of bipA alleles within and across Bordetella species.
Infect Immun. 2003 Jun;71(6):3043-52. doi: 10.1128/IAI.71.6.3043-3052.2003.
9
Role of antibodies in immunity to Bordetella infections.
Infect Immun. 2003 Apr;71(4):1719-24. doi: 10.1128/IAI.71.4.1719-1724.2003.
10
Lipopolysaccharide endotoxins.
Annu Rev Biochem. 2002;71:635-700. doi: 10.1146/annurev.biochem.71.110601.135414. Epub 2001 Nov 9.
本文引用的文献
1
A porcine model for the evaluation of virulence of Bordetella bronchiseptica.
J Comp Pathol. 1997 Jan;116(1):55-61. doi: 10.1016/s0021-9975(97)80043-6.
2
Economic impact of pertussis.
Arch Pediatr Adolesc Med. 1997 Jan;151(1):35-40. doi: 10.1001/archpedi.1997.02170380039006.
3
Bacterial polysaccharide synthesis and gene nomenclature.
Trends Microbiol. 1996 Dec;4(12):495-503. doi: 10.1016/s0966-842x(97)82912-5.
4
Treatment of acute bronchitis in adults without underlying lung disease.
J Gen Intern Med. 1996 Sep;11(9):557-62. doi: 10.1007/BF02599608.
5
Moredun Bordetella Medium, an improved selective medium for isolation of Bordetella parapertussis.
J Clin Microbiol. 1996 Mar;34(3):638-40. doi: 10.1128/jcm.34.3.638-640.1996.
6
Historical review of pertussis and the classical vaccine.
J Infect Dis. 1996 Nov;174 Suppl 3:S259-63. doi: 10.1093/infdis/174.supplement_3.s259.
7
The identification, cloning and mutagenesis of a genetic locus required for lipopolysaccharide biosynthesis in Bordetella pertussis.
Mol Microbiol. 1996 Jan;19(1):37-52. doi: 10.1046/j.1365-2958.1996.354877.x.
8
Mutational analysis and properties of the msbA gene of Escherichia coli, coding for an essential ABC family transporter.
Mol Microbiol. 1996 Jun;20(6):1221-33. doi: 10.1111/j.1365-2958.1996.tb02642.x.
9
Bordetella bronchoseptica pneumonia with shock in an immunocompetent patient.
Scand J Infect Dis. 1996;28(2):197-8. doi: 10.3109/00365549609049077.
10
The differentiation of Bordetella parapertussis and Bordetella bronchiseptica from humans and animals as determined by DNA polymorphism mediated by two different insertion sequence elements suggests their phylogenetic relationship.
Int J Syst Bacteriol. 1996 Jul;46(3):640-7. doi: 10.1099/00207713-46-3-640.
Zotero 插件