Olmarker K, Brisby H, Yabuki S, Nordborg C, Rydevik B
Department of Orthopaedics, University of Gothenburg, Sahlgrenska University Hospital, Sweden.
Spine (Phila Pa 1976). 1997 Mar 1;22(5):471-5; discussion 476. doi: 10.1097/00007632-199703010-00001.
Autologous nucleus pulposus was modified and applied to the cauda equina in pigs. Histology and neurophysiology were assessed after 7 days.
To assess if alterations of the nucleus pulposus would change the degree and distribution of the nerve injury induced by autologous nucleus pulposus.
It was reported recently that nucleus pulposus may induce structural and functional changes in nerve roots after epidural application. The basic mechanisms causing these changes are not fully understood.
Nucleus pulposus was harvested from lumbar discs and submitted to either of three treatments; 37 C for 24 hours (n = 5), -20 C for 24 hours (n = 5), or digestion by hyaluronidase for 24 hours (n = 6). In two additional pigs, nucleus pulposus was applied just after harvest as a control to verify previous observations. After 7 days, nerve conduction velocity was recorded, and specimens were processed for blinded light microscopic assessment.
When nucleus pulposus was applied just after harvest, or when it had been kept at 37 C or digested by hyaluronidase for 24 hours, there was a significant reduction in nerve conduction velocity similar to previous observations. When nucleus pulposus had been kept at -20 C for 24 hours, however, there was no reduction in conduction velocity. There were no apparent differences between the groups at the histologic assessment. Staining of the nucleus pulposus showed that the cells in the nucleus pulposus exposed to -20 C were lysed, whereas the cells in the nucleus pulposus treated by the two other methods were mainly unaffected.
Because freezing of the nucleus pulposus probably kills the cells but does not affect other components, one may assume that the biologic effects induced by the nucleus pulposus may be related to its cell population.
将自体髓核进行改良后应用于猪的马尾神经。7天后评估组织学和神经生理学情况。
评估髓核的改变是否会改变自体髓核所致神经损伤的程度和分布。
最近有报道称，硬膜外应用髓核可能会引起神经根的结构和功能改变。导致这些改变的基本机制尚未完全明确。
从腰椎间盘获取髓核，并进行三种处理之一；37℃处理24小时（n = 5），-20℃处理24小时（n = 5），或用透明质酸酶消化24小时（n = 6）。另外两只猪在获取髓核后立即应用作为对照，以验证之前的观察结果。7天后，记录神经传导速度，并对标本进行盲法光学显微镜评估。
当髓核在获取后立即应用，或在37℃保存或用透明质酸酶消化24小时后应用时，神经传导速度显著降低，与之前的观察结果相似。然而，当髓核在-20℃保存24小时后，传导速度没有降低。组织学评估时各组之间没有明显差异。髓核染色显示，暴露于-20℃的髓核细胞溶解，而用其他两种方法处理的髓核细胞基本未受影响。
由于髓核冷冻可能会杀死细胞但不影响其他成分，因此可以推测髓核诱导的生物学效应可能与其细胞群体有关。
