Olmarker K, Larsson K
Department of Orthopaedics, Sahlgrenska University Hospital, Göteborg University, Sweden.
Spine (Phila Pa 1976). 1998 Dec 1;23(23):2538-44. doi: 10.1097/00007632-199812010-00008.
The effects of nucleus pulposus and various treatments to block tumor necrosis factor alpha activity were evaluated in an experimental set-up using immunohistochemistry and nerve conduction velocity recordings.
To assess the presence of tumor necrosis factor alpha in pig nucleus pulposus cells, and to see if block of tumor necrosis factor alpha also blocks the nucleus-pulposus-induced reduction of nerve root conduction velocity.
A meta-analysis of observed effects induced by nucleus pulposus revealed that these effects might relate to one specific cytokine-tumor necrosis factor alpha.
Series-1: Cultured nucleus pulposus cells were stained immunohistologically with a monoclonal antibody for tumor necrosis factor alpha. Series-2: Nucleus pulposus was harvested from lumbar discs and applied to the sacrococcygeal cauda equina in 13 pigs autologously. Four pigs received 100 mg of doxycycline intravenously; five pigs had a blocking monoclonal antibody to tumor necrosis factor alpha applied locally in the nucleus pulposus, and four pigs remained nontreated, forming a control group. Three days after the application, the nerve root conduction velocity was determined over the application zone by local electrical stimulation.
Series-1: Tumor necrosis factor alpha was found to be present in the nucleus pulposus cells. Series-2: The selective antibody to tumor necrosis factor alpha limited the reduction of nerve conduction velocity, although in comparison with the control group this was not statistically significant. However, treatment with doxycycline significantly blocked the nucleus-pulposus-induced reduction of conduction velocity.
For the first time, a specific substance, tumor necrosis factor alpha, has been linked to the nucleus-pulposus-induced effects of nerve roots after local application. Although the effects of this substance may be synergistic with those of other similar substances, the data of the current study may be of significant importance for the continued understanding of nucleus pulposus' biologic activity, and of possible potential use for future strategies in managing sciatica.
在一个实验装置中，使用免疫组织化学和神经传导速度记录来评估髓核以及各种阻断肿瘤坏死因子α活性的治疗方法的效果。
评估猪髓核细胞中肿瘤坏死因子α的存在情况，并观察阻断肿瘤坏死因子α是否也能阻断髓核诱导的神经根传导速度降低。
对髓核诱导的观察效应进行的荟萃分析表明，这些效应可能与一种特定的细胞因子——肿瘤坏死因子α有关。
系列1：用针对肿瘤坏死因子α的单克隆抗体对培养的髓核细胞进行免疫组织化学染色。系列2：从腰椎间盘采集髓核，并将其自体应用于13头猪的骶尾马尾神经。4头猪静脉注射100毫克强力霉素；5头猪在髓核局部应用针对肿瘤坏死因子α的阻断单克隆抗体，4头猪不进行治疗，作为对照组。应用后三天，通过局部电刺激在应用区域测定神经根传导速度。
系列1：发现肿瘤坏死因子α存在于髓核细胞中。系列2：肿瘤坏死因子α的选择性抗体限制了神经传导速度的降低，尽管与对照组相比，这在统计学上不显著。然而，强力霉素治疗显著阻断了髓核诱导的传导速度降低。
首次将一种特定物质——肿瘤坏死因子α与局部应用髓核后对神经根的诱导效应联系起来。尽管这种物质的效应可能与其他类似物质的效应协同，但本研究的数据对于持续理解髓核的生物活性以及未来治疗坐骨神经痛策略的潜在应用可能具有重要意义。
