使用放射免疫沉淀-聚乙二醇测定法（RIPEGA）检测抗IgG抗体。
The use of the radioimmunoprecipitation-PEG assay (RIPEGA) to detect anti-IgG antibodies.
作者信息
Santoro F, Duquesnoy B, Wattre P, Delcambre B, Capron A
出版信息
Biomedicine. 1977 Jul;27(6):230-3.
A new procedure, the radioimmunoprecipitation--P.E.G. assay (RIPEGA) is proposed for the detection of anti-IgG antibodies (anti-IgG Ab). This sensitive and reproducible test was performed by incubation of radio-labelled human IgG (125I-IgG) with patient's sera. Separation of free from complexed 125I-IgG was achieved by a 7% P.E.G. precipitation. Preliminary results demonstrated anti-IgG Ab in 84.4% of the cases of sero-positive rheumatoid arthritis (R.A.), 85% of seronegative R.A., 43% of other forms of inflammatory arthritis, 50% of unclassified arthritis and in 10.5% of the control subjects.
一种新的检测抗IgG抗体（抗IgG Ab）的方法——放射免疫沉淀-聚乙二醇检测法（RIPEGA）被提出来了。这种灵敏且可重复的检测是通过将放射性标记的人IgG（125I-IgG）与患者血清孵育来进行的。通过7%的聚乙二醇沉淀实现游离的125I-IgG与结合的125I-IgG的分离。初步结果显示，在血清阳性类风湿关节炎（R.A.）病例中，84.4%存在抗IgG Ab；血清阴性R.A.病例中，85%存在；其他形式的炎性关节炎病例中，43%存在；未分类关节炎病例中，50%存在；而在对照受试者中，10.5%存在。
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