Solé F, Woessner S, Pérez-Losada A, Florensa L, Montero S, Asensio A, Besses C, Sans-Sabrafen J
Laboratori de Citologia Hematológica, Hospital Central L' Aliança, Barcelona, Spain.
Cancer Genet Cytogenet. 1997 Feb;93(2):160-6. doi: 10.1016/s0165-4608(96)00191-4.
The results of cytogenetic studies are reported in 76 patients with B-chronic lymphoproliferative disorders (B-CLPD): 60 patients with chronic lymphocytic leukemia (CLL), six with follicular lymphoma in leukemic phase (FLLP), five with splenic B-cell lymphoma with villous lymphocytes (SLVL), two with chronic prolymphocytic leukemia (CPL), two with hairy cell leukemia (HCL), and one with plasma cell leukemia (PCL). PHA (phytohemagglutinin), PWM (pokeweed mitogen), LPS (lipopolysaccharide from Escherichia Coli), TPA (phorbol 12-myristate acetate), IL6 (interleukin 6), and DxS (dextran sulfate) were used as mitogens. Mitoses were obtained in 75 cases. Clonal aberrations could be demonstrated in 34 cases (44%). In CLL, classical type, chromosomes 6, 11, and 13 were more frequently involved, whereas trisomy 12 was frequently found in CLL mixed-cell type, in FLLP, and CPL. In SLVL the deletion del(7)(q32) is noteworthy and miscellaneous chromosome abnormalities in the remaining patients were observed. Regarding the efficiency of mitogens, PHA turned to be the most effective in obtaining metaphases and in detecting clonal chromosomal aberrations.
报告了76例B细胞慢性淋巴细胞增殖性疾病（B-CLPD）患者的细胞遗传学研究结果：60例慢性淋巴细胞白血病（CLL）患者、6例白血病期滤泡性淋巴瘤（FLLP）患者、5例脾B细胞淋巴瘤伴绒毛淋巴细胞（SLVL）患者、2例慢性原淋巴细胞白血病（CPL）患者、2例毛细胞白血病（HCL）患者和1例浆细胞白血病（PCL）患者。使用植物血凝素（PHA）、商陆有丝分裂原（PWM）、脂多糖（LPS，来自大肠杆菌）、佛波酯（TPA，12-肉豆蔻酸佛波酯）、白细胞介素6（IL6）和硫酸葡聚糖（DxS）作为有丝分裂原。75例患者获得了有丝分裂相。34例（44%）可证明存在克隆性畸变。在典型类型的CLL中，6号、11号和13号染色体更常受累，而在CLL混合细胞型、FLLP和CPL中常发现12号染色体三体。在SLVL中，del(7)(q32)缺失值得注意，其余患者观察到各种染色体异常。关于有丝分裂原效率，PHA在获得中期相和检测克隆性染色体畸变方面是最有效的。
