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植物中的细胞核组织

Nuclear organization in plants.

作者信息

Shaw P J

机构信息

Department of Cell Biology, John Innes Centre, Colney, Norwich, U.K.

出版信息

Essays Biochem. 1996;31:77-89.

PMID:9078459
Abstract

Plant nuclei, like animal nuclei, are highly organized organelles, and much evidence is now accumulating that their organization is central to their function. Many plant nuclei are amenable to detailed structural analysis because of their large size, and the economic importance of plants as crops makes it important to understand the functional organization of plant nuclei, in particular gene expression. Problems such as gene silencing will probably require an understanding of nuclear organization for their solution. Several identifiable domains are now known in the nucleus, including the nucleolus, coiled and other nuclear bodies, and interchromatin granule clusters, but much of their function remains obscure. The nucleolus may be regarded as a giant transcription and transcript-processing domain. The steps of ribosome biogenesis are organized within it spatially as well as temporally. It may also provide a useful model for other nuclear transcription domains.

摘要

植物细胞核与动物细胞核一样,是高度有序的细胞器,目前越来越多的证据表明其结构对于其功能至关重要。许多植物细胞核由于体积较大,适合进行详细的结构分析,而且植物作为农作物具有经济重要性,这使得了解植物细胞核的功能组织,尤其是基因表达变得很重要。诸如基因沉默等问题可能需要了解核组织才能解决。目前已知细胞核中有几个可识别的区域,包括核仁、卷曲体和其他核体以及染色质间颗粒簇,但它们的许多功能仍不清楚。核仁可被视为一个巨大的转录和转录加工区域。核糖体生物发生的步骤在其中在空间和时间上都是有组织的。它也可能为其他核转录区域提供一个有用的模型。

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Dynamic interactions between splicing snRNPs, coiled bodies and nucleoli revealed using snRNP protein fusions to the green fluorescent protein.利用与绿色荧光蛋白融合的snRNP蛋白揭示剪接snRNP、卷曲小体和核仁之间的动态相互作用。
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Differentiating plant cells switched to proliferation remodel the functional organization of nuclear domains.转变为增殖状态的分化植物细胞会重塑核域的功能组织。
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引用本文的文献

1
The Multiple Functions of the Nucleolus in Plant Development, Disease and Stress Responses.核仁在植物发育、疾病和应激反应中的多重功能
Front Plant Sci. 2018 Feb 9;9:132. doi: 10.3389/fpls.2018.00132. eCollection 2018.
2
Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families.方案:优化茄科和蔷薇科植物叶片细胞核分离方法。
Plant Methods. 2013 Jul 26;9:31. doi: 10.1186/1746-4811-9-31. eCollection 2013.
3
Nuclear bodies and compartmentalization of pre-mRNA splicing factors in higher plants.
高等植物中的核小体与前体mRNA剪接因子的区室化
Chromosoma. 2004 Feb;112(5):255-66. doi: 10.1007/s00412-003-0271-3. Epub 2004 Jan 23.
4
The ROOT HAIRLESS 1 gene encodes a nuclear protein required for root hair initiation in Arabidopsis.根无毛1基因编码拟南芥根毛起始所需的一种核蛋白。
Genes Dev. 1998 Jul 1;12(13):2013-21. doi: 10.1101/gad.12.13.2013.