爱泼斯坦-巴尔病毒基因组在一个嵌套的内部开放阅读框中编码脱氧胸苷激酶活性。

The Epstein-Barr virus genome encodes deoxythymidine kinase activity in a nested internal open reading frame.

作者信息

Holton R H, Gentry G A

机构信息

Department of Microbiology, University of Mississippi Medical Center, Jackson 39216-4505, USA.

出版信息

Intervirology. 1996;39(4):270-4. doi: 10.1159/000150528.

DOI:10.1159/000150528

PMID:9078468

Abstract

The Epstein-Barr virus (EBV) BXLF1 fragment open reading frame (LORF), though to encode deoxythymidine kinase (dTK) activity, and a shorter frame (SORF), starting at an internal in-frame AUG, were isolated by polymerase chain reaction from a plasmid containing the EcoR1 fragment of EBV strain FF-41. These were transfected into dTK-Escherichia coli, producing multiple SORF- or LORF-containing colonies, which expressed dTK. The 243 NH2-terminal residues of the LORF-encoded polypeptide thus are not essential for dTK activity. SORF, with 1,092 bp, is predicted to encode a 36- to 37-kD polypeptide, in the size range of other herpesviral dTKs.

摘要

爱泼斯坦-巴尔病毒（EBV）BXLF1片段开放阅读框（LORF），虽被认为可编码脱氧胸苷激酶（dTK）活性，以及一个起始于框内内部AUG的较短阅读框（SORF），通过聚合酶链反应从含有EBV FF-41株EcoR1片段的质粒中分离得到。将它们转染到dTK缺陷型大肠杆菌中，产生了多个含有SORF或LORF的菌落，这些菌落表达dTK。因此，LORF编码的多肽的243个氨基末端残基对于dTK活性并非必需。SORF长度为1092 bp，预计可编码一种36至37 kD的多肽，处于其他疱疹病毒dTKs的大小范围内。