Telford E A, Watson M S, Aird H C, Perry J, Davison A J
Institute of Virology, University of Glasgow, UK.
J Mol Biol. 1995 Jun 9;249(3):520-8. doi: 10.1006/jmbi.1995.0314.
The complete DNA sequence of equine herpesvirus 2 (EHV-2) strain 86/67 was determined. The genome is 184,427 bp in size and has a base composition of 57.5% G + C. Unusually for a herpesvirus, about a third of the sequence distributed in several large blocks appears not to encode proteins. The 79 open reading frames that were identified as probably polypeptide-coding are predicted to encode 77 distinct proteins. Amino acid sequence comparisons confirmed that EHV-2 is a gamma-herpesvirus that is genetically collinear with herpesvirus saimiri (HVS; a gamma 2-herpesvirus) and Epstein-Barr virus (EBV; a gamma 1-herpesvirus), with a closer relationship to the former. Moreover, EHV-2 specifies eight proteins that have counterparts in HVS but not in EBV and only a single protein that has a homologue in EBV but not in HVS (EBV BCRF1, which encodes an interleukin 10-like protein). EHV-2 also encodes three potential G protein-coupled receptors, one with a counterpart in HVS that is specific for alpha chemokines, another with a counterpart in human cytomegalovirus (a beta-herpesvirus), which is specific for beta chemokines, and a third that is assigned more tentatively and lacks detectable counterparts in other herpesviruses.
测定了马疱疹病毒2型（EHV - 2）86/67株的完整DNA序列。该基因组大小为184,427碱基对，碱基组成为57.5%的G + C。对于疱疹病毒而言不同寻常的是，分布在几个大区域中的约三分之一序列似乎不编码蛋白质。已鉴定出的79个可能编码多肽的开放阅读框预计编码77种不同的蛋白质。氨基酸序列比较证实，EHV - 2是一种γ疱疹病毒，在基因上与猴疱疹病毒（HVS；一种γ2疱疹病毒）和爱泼斯坦 - 巴尔病毒（EBV；一种γ1疱疹病毒）呈线性关系，与前者关系更密切。此外，EHV - 2有8种蛋白质在HVS中有对应物，但在EBV中没有，只有一种蛋白质在EBV中有同源物，但在HVS中没有（EBV BCRF1，其编码一种白细胞介素10样蛋白）。EHV - 2还编码三种潜在的G蛋白偶联受体，一种在HVS中有对应物，对α趋化因子具有特异性，另一种在人巨细胞病毒（一种β疱疹病毒）中有对应物，对β趋化因子具有特异性，第三种的归属较为暂定，在其他疱疹病毒中缺乏可检测到的对应物。
