Domínguez M G, Rivera H, Troyo R
División de Genética, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, México.
Arch Med Res. 1997 Spring;28(1):25-7.
We evaluated the effect of different concentrations of colchicine (0.0, 0.1, 0.3, 0.5 microgram/ml) and phytohemagglutinin (PHA) (0.10, 0.15, 0.20 microgram/ml) on the rate of C-anaphases in lymphocyte cultures from five healthy individuals with the common variant of C-anaphases. For each of the 12 possible combinations, two subjects were randomly tested. The frequency of these variant figures was < 3%; a single-culture (out of six with the colchicine concentration of 0.1 microgram/ml) lacked C-anaphases. Multiple variance and Student's t tests revealed, as the only significant difference, a decrease with the colchicine concentration of 0.3 microgram/ml compared with the cultures without colchicine (p < 0.05), which exhibited the greatest ratio of C-anaphases. The PHA had no influence on the frequency of C-anaphases. We conclude that the common variant of C-anaphases is unrelated to the colchicine and PHA concentrations tested; moreover, our data confirm the occurrence of such a mitotic variant in colchicine-free cultures.
我们评估了不同浓度的秋水仙碱（0.0、0.1、0.3、0.5微克/毫升）和植物血凝素（PHA）（0.10、0.15、0.20微克/毫升）对五名具有常见C后期变体的健康个体淋巴细胞培养物中C后期发生率的影响。对于12种可能的组合中的每一种，随机测试两名受试者。这些变体图形的频率<3%；在秋水仙碱浓度为0.1微克/毫升的六次培养中，有一次单一培养缺乏C后期。多变量分析和学生t检验显示，唯一显著的差异是，与未添加秋水仙碱的培养物相比，秋水仙碱浓度为0.3微克/毫升时C后期发生率降低（p<0.05），未添加秋水仙碱的培养物中C后期发生率最高。PHA对C后期的频率没有影响。我们得出结论，常见的C后期变体与所测试的秋水仙碱和PHA浓度无关；此外，我们的数据证实了在无秋水仙碱的培养物中存在这种有丝分裂变体。
