Naito Y, Ohtawara Y, Kageyama S, Nakano M, Ichiyama A, Fujita M, Suzuki K, Kawabe K, Kino I
First Department of Pathology, Hamamatsu University School of Medicine, Japan.
Urol Res. 1997;25(1):59-65. doi: 10.1007/BF00941907.
Cell culture models of calcium phosphate renal stone formation were established using the MDCK cell line. Renal microliths were detected within pseudocysts in three-dimensional soft agar cultures, and were also observed in the basal region of cells lining the cell sheet, and immediately beneath domes or blisters in monolayers and collagen gel cultures. Light and scanning electron microscopy indicated that these microliths had a similar lamellated and spherical appearance to those in humans. These microliths were first detected microscopically after 21 days of culture, and were found to be composed of calcium phosphate by X-ray and micro-infrared spectroscopic analyses. These culture models may provide a powerful new tool to study the pathogenesis of renal stone diseases and/or calcium phosphate stone formation in humans and animals.
利用MDCK细胞系建立了磷酸钙肾结石形成的细胞培养模型。在三维软琼脂培养物的假囊肿内检测到肾微结石，在细胞单层和胶原凝胶培养物中，细胞片层内衬细胞的基底区域以及穹顶或水泡正下方也观察到了肾微结石。光学显微镜和扫描电子显微镜显示，这些微结石的层状和球形外观与人类的相似。这些微结石在培养21天后首次通过显微镜检测到，经X射线和微红外光谱分析发现其由磷酸钙组成。这些培养模型可能为研究人类和动物肾结石疾病的发病机制和/或磷酸钙结石形成提供一个强大的新工具。
