Whang K S, Whang C W
Department of Chemistry, Tunghai University, Taichung, Taiwan.
Electrophoresis. 1997 Feb;18(2):241-6. doi: 10.1002/elps.1150180211.
A capillary electrophoresis (CE)-indirect UV absorbance detection method for organotin compounds is described. The five triorganotin cations, viz., trimethyltin (TMT), triethyltin (TET), tripropyltin (TPT), tributyltin (TBT) and triphenyltin (TPhT), were efficiently separated by CE in 10 min with a 5 mM acetate buffer (pH 4.5) containing 3 mM 4-aminopyridine (4-AP). 4-AP also functioned as a UV-absorbing coion for the indirect detection of these UV-transparent organotin species. Simultaneous separation of di- and triorganotins was achieved with the addition of alpha-cyclodextrin (alpha-CD) as a modifier in the electrophoresis buffer. Linearity (r > or = 0.995) of about two orders of magnitude was generally obtained. The concentration limits of detection for the organotin compounds studied were between 2 and 20 microM (as tin). The relative standard deviation (RSD) values were in the range 1.3-7.1%. The applicability of the method for the analysis of marine sediment was examined.
描述了一种用于有机锡化合物的毛细管电泳（CE）-间接紫外吸收检测方法。五种三有机锡阳离子，即三甲基锡（TMT）、三乙基锡（TET）、三丙基锡（TPT）、三丁基锡（TBT）和三苯基锡（TPhT），在含有3 mM 4-氨基吡啶（4-AP）的5 mM醋酸盐缓冲液（pH 4.5）中，通过CE在10分钟内有效分离。4-AP还用作紫外吸收共离子，用于间接检测这些紫外透明的有机锡物种。通过在电泳缓冲液中添加α-环糊精（α-CD）作为改性剂，实现了二有机锡和三有机锡的同时分离。通常获得约两个数量级的线性（r≥0.995）。所研究的有机锡化合物的检测浓度限在2至20μM（以锡计）之间。相对标准偏差（RSD）值在1.3-7.1%范围内。考察了该方法在海洋沉积物分析中的适用性。
