Key Laboratory of Analysis and Detection Technology for Food Safety of Ministry of Education, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
Talanta. 2010 Mar 15;80(5):1913-8. doi: 10.1016/j.talanta.2009.10.043. Epub 2009 Oct 24.
A microwave-assisted extraction used to extract trace triorganotin from aquatic organisms and a sensitive analytical method for the determination of ultratrace triorganotin (namely trimethyltin, triethyltin, tripropyltin and tributyltin) with capillary electrophoresis-inductively coupled plasma mass spectrometry were firstly described in this study. The extraction method is simple, effective and can be used to extract trace triorganotin in aquatic organisms within several min. The analytical method has a much lower detection limit of 0.2-0.7 ng Sn/mL for triorganotin compounds, and can be used to determine trace triorganotin in aquatic organisms directly without any derivatization and preconcentration. Using above methods, we have successfully determined trimethyltin, triethyltin, tripropyltin and tributyltin in dried Mya arenaria Linnaeus and Corbicula fluminea within 17 min with a recovery of 93-104% and a RSD (relative standard deviation, n=6) of 2-5%. Our results showed that dried M. arenaria Linnaeus contained an extremely high tributyltin of 5.1 microg Sn/g dried weight, indicating that it may be a good biomarker for the organotin pollution in ocean.
本研究首次描述了一种微波辅助提取方法，用于从水生生物中提取痕量三有机锡，以及一种使用毛细管电泳-电感耦合等离子体质谱法测定痕量三有机锡（即三甲基锡、三乙基锡、三丙基锡和三丁基锡）的灵敏分析方法。该提取方法简单、有效，可在几分钟内提取水生生物中的痕量三有机锡。分析方法对三有机锡化合物的检测限低至 0.2-0.7ngSn/mL，可直接用于测定水生生物中的痕量三有机锡，无需衍生化和预浓缩。使用上述方法，我们成功地在 17 分钟内测定了干贻贝和河蚬中的三甲基锡、三乙基锡、三丙基锡和三丁基锡，回收率为 93-104%，相对标准偏差（n=6）为 2-5%。研究结果表明，干贻贝中含有极高的三丁基锡，达 5.1μgSn/g 干重，表明其可能是海洋有机锡污染的良好生物标志物。
