Jeyendran R S, Gunawardana V K, Barisic D, Wentz A C
Andrology Laboratory Services, Inc., Chicago, IL 60611, USA.
Hum Reprod Update. 1995 Jan;1(1):73-9. doi: 10.1093/humupd/1.1.73.
Media prepared with egg yolk and two buffers, TES and Tris, called TEST-yolk have been shown to have beneficial effects on the survivability, fertilizing capacity and storage potential of human spermatozoa. Egg yolk lipoproteins are the critical compounds for the beneficial effects, with a synergistic effect due to the TES-Tris buffer component. TEST-yolk media have been used in the sperm penetration assay, the hemizona assay, sperm preparation for clinical in-vitro fertilization, artificial insemination with homologous spermatozoa, cryopreservation, sperm samples with a positive antisperm antibody test, and preparation for techniques requiring capacitated spermatozoa. Few harmful consequences of TEST-yolk have been reported, although controlled trials are required to evaluate therapeutic effects in the treatment of male infertility.
用蛋黄以及两种缓冲液（TES和Tris）配制的培养基，即TEST - 蛋黄培养基，已被证明对人类精子的存活率、受精能力和储存潜力具有有益作用。蛋黄脂蛋白是产生有益作用的关键化合物，由于TES - Tris缓冲液成分具有协同效应。TEST - 蛋黄培养基已用于精子穿透试验、半透明带试验、临床体外受精的精子制备、同种异体精子人工授精、冷冻保存、抗精子抗体检测呈阳性的精子样本，以及需要获能精子的技术准备。尽管需要进行对照试验来评估其对男性不育治疗的疗效，但关于TEST - 蛋黄培养基的有害后果报道较少。
