Rosenthal K L, Tompkins W A, Frank G L, McCulloch P, Rawls W E
Cancer Res. 1977 Nov;37(11):4024-30.
A variant, HCT-8R, of the colon adenocarcinoma cell line HCT-8 was located and cloned. The variant and parent cell line were characterized with respect to morphology, growth characteristics, karyotype, production of Cea, and ability to form tumors in nude mice. The variant cells differed from the parent cells in morphology, marker chromosomes, and ability to form colonies in soft agar and produced more carcinoembryonic antigen. The two cell strains were equally oncogenic in nude mice, although HCT-8R cells produced poorly differentiated tumors while HCT-8 cells produced tumors with both differentiated and poorly differentiated areas. Thus, in nude mice, no correlation was observed between carcinoembryonic antigen production by cells and their oncogenicity.
一种结肠腺癌细胞系HCT - 8的变体HCT - 8R被定位并克隆。对该变体和亲本细胞系在形态、生长特性、核型、癌胚抗原的产生以及在裸鼠中形成肿瘤的能力等方面进行了表征。变体细胞在形态、标记染色体以及在软琼脂中形成集落的能力方面与亲本细胞不同，并且产生更多的癌胚抗原。这两种细胞株在裸鼠中具有同等的致癌性，尽管HCT - 8R细胞产生低分化肿瘤，而HCT - 8细胞产生的肿瘤既有分化区域也有低分化区域。因此，在裸鼠中，未观察到细胞产生癌胚抗原与其致癌性之间的相关性。
