Bosman G J, Renkawek K, Reijnen-Aalbers A, De Grip W J
Department of Biochemistry, University of Nijmegen, The Netherlands.
Neuroreport. 1997 Jan 20;8(2):427-30. doi: 10.1097/00001756-199701200-00010.
We used primary cultures of rat hippocampal tissue to estimate the contribution of anion exchange (AE) proteins to the regulation of intracellular pH in neurones and astrocytes. After induction of acidosis, neonatal rat astrocytes were able to restore the intracellular pH in the absence of extracellular bicarbonate. Neonatal neurones, however, were able to recover from acidosis only when bicarbonate was present in the extracellular medium. This recovery was inhibited by inhibition of anion exchange and was independent of the presence of sodium ions. Antibodies against AE proteins reacted predominantly with neurones. These data suggest that neurones in particular are dependent on functional AE proteins for the maintenance of their intracellular pH.
我们使用大鼠海马组织的原代培养物来评估阴离子交换（AE）蛋白对神经元和星形胶质细胞内pH调节的贡献。诱导酸中毒后，新生大鼠星形胶质细胞能够在细胞外无碳酸氢盐的情况下恢复细胞内pH。然而，新生神经元只有在细胞外培养基中存在碳酸氢盐时才能从酸中毒中恢复。这种恢复受到阴离子交换抑制的影响，且与钠离子的存在无关。针对AE蛋白的抗体主要与神经元发生反应。这些数据表明，特别是神经元维持其细胞内pH依赖于功能性AE蛋白。
