Pironcheva G L, Russev G
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Cytobios. 1996;86(347):243-6.
The level of transfection of myoblast C-2/C-12 cells with the plasmid PSV40-beta-globin, using the immunofluorescence assay, was investigated. Myoblasts, which are non-terminally differentiated cells, were chosen since it is known that terminally differentiated myotubes are very poorly transfected. The results showed that myoblast cells of the C-2/C-12 cell line were readily transfected within 24 h with the PSV40-beta-globin gene. The immunofluorescence assay showed the nucleoplasmic localization of the SV40 large T antigen, and indicated the possibility that some fraction of it might be localized in the cytoplasm.
利用免疫荧光测定法，研究了质粒PSV40-β-珠蛋白对成肌细胞C-2/C-12的转染水平。选择成肌细胞作为研究对象，因为它们是未终末分化的细胞，而已知终末分化的肌管转染效率很低。结果显示，C-2/C-12细胞系的成肌细胞在24小时内即可被PSV40-β-珠蛋白基因成功转染。免疫荧光测定显示SV40大T抗原定位于核质，并且表明其一部分可能定位于细胞质中。
