Pironcheva G L, Russev G
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Microbios. 1997;89(358):31-8.
The relationship between preferences among alternative 5' splice sites and their sequences was investigated using as model mouse myoblasts and myotubes after transient transfection with the rabbit beta-globin gene. The preferences for the use of two different 5' splice sites, acting with different efficiencies to direct splicing in vivo are reported. The predominant selection of the upstream splice site has been shown in normal mouse myoblasts. In the case of differentiated myotubes the downstream splice was 1.4 times better used. The results indicate that there were differences in the preferences for the use of the two alternative splice sites between non-differentiated and terminally differentiated cells, within the same-cell line.
利用兔β-珠蛋白基因瞬时转染后的小鼠成肌细胞和肌管作为模型，研究了5'剪接位点的不同选择与其序列之间的关系。报道了两种不同5'剪接位点在体内指导剪接时效率不同的使用偏好。在正常小鼠成肌细胞中已显示上游剪接位点的优先选择。在分化的肌管中，下游剪接的使用效果要好1.4倍。结果表明，在同一细胞系中，未分化细胞和终末分化细胞对两种可变剪接位点的使用偏好存在差异。
