Wan J, Gordon J B, Muirhead K, Hickey M W, Coventry M J
Australian Food Industry Science Centre, Werribee, Victoria, Australia.
Lett Appl Microbiol. 1997 Mar;24(3):153-8. doi: 10.1046/j.1472-765x.1997.00294.x.
Nisin was successfully incorporated into a matrix of calcium alginate and ground into micro-particles smaller than 150 microns. Formation of micro-particles and incorporation of nisin was verified by scanning electron microscopy and by the reduction in the inactivation of nisin activity with proteolytic enzymes. Incorporation efficiency was 87-93% and the nisin in the alginate-incorporated form was 100% active against an indicator culture of Lactobacillus curvatus both in MRS broth and reconstituted skim milk.
乳链菌肽成功地被包埋在海藻酸钙基质中，并研磨成小于150微米的微粒。通过扫描电子显微镜以及用蛋白水解酶处理后乳链菌肽活性失活的降低情况，验证了微粒的形成以及乳链菌肽的包埋。包埋效率为87%-93%，在MRS肉汤和复原脱脂乳中，包埋在海藻酸盐中的乳链菌肽对弯曲乳杆菌指示培养物的活性均为100%。
