Chumchalová J, Josephsen J, Plocková M
Department of Dairy and Fat Technology, Institute of Chemical Technology, Prague, Czech Republic.
Int J Food Microbiol. 1998 Aug 18;43(1-2):33-8. doi: 10.1016/s0168-1605(98)00094-4.
The ability of acidocin CH5, a bacteriocin from Lactobacillus acidophilus CH5 in the form of neutralized and heated supernatant, to prevent the growth of the indicator Lactobacillus delbrueckii subsp. lactis LTI 30 alone or together with other antimicrobials was investigated. The inhibitory activity of acidocin CH5 was higher in MRS broth than in reconstituted skim milk (RSM). In MRS broth and RSM, 1.92 and 32 AU acidocin CH5/ml, respectively, caused 97 and 89% inhibition of the indicator Lactobacillus delbrueckii subsp. lactis LTI 30. The presence of 5 and 10% milk fat in RSM decreased the inhibitory activity of acidocin CH5 to 20 and 11%, respectively. The inhibitory activity of acidocin CH5 was also reduced in the presence of NaCl, NaNO3 and lysozyme. In RSM the inhibition was weaker with both acidocin CH5 and NaCl added compared with NaCl alone. In MRS broth the inhibition was stronger with both acidocin CH5 and NaCl added compared with NaCl alone. The inhibition of the indicator Lactobacillus delbrueckii subsp. lactis LTI 30 was stronger with both NaNO3 and acidocin CH5 in MRS broth (but not in RSM) than with only NaNO3 present, but the strongest level was obtained with acidocin CH5 alone. Addition of acidocin CH5 and more than 30 mg/ml lysozyme to MRS broth increased the level of inhibition above the level obtained by acidocin CH5 alone. The indicator Lactobacillus delbrueckii subsp. lactis LTI 30 was also sensitive to NaCl, NaNO3 and lysozyme in both MRS broth and RSM.
嗜酸乳杆菌CH5产生的细菌素嗜酸菌素CH5以中和并加热的上清液形式存在，研究了其单独或与其他抗菌剂一起阻止指示菌德氏乳杆菌乳酸亚种LTI 30生长的能力。嗜酸菌素CH5在MRS肉汤中的抑制活性高于在复原脱脂乳（RSM）中的抑制活性。在MRS肉汤和RSM中，分别为1.92和32 AU/ml的嗜酸菌素CH5对指示菌德氏乳杆菌乳酸亚种LTI 30产生了97%和89%的抑制作用。RSM中存在5%和10%的乳脂肪时，嗜酸菌素CH5的抑制活性分别降至20%和11%。在NaCl、NaNO₃和溶菌酶存在的情况下，嗜酸菌素CH5的抑制活性也会降低。在RSM中，与单独添加NaCl相比，同时添加嗜酸菌素CH5和NaCl时抑制作用较弱。在MRS肉汤中，与单独添加NaCl相比，同时添加嗜酸菌素CH5和NaCl时抑制作用更强。在MRS肉汤中（但不在RSM中），与仅存在NaNO₃相比，同时存在NaNO₃和嗜酸菌素CH5时对指示菌德氏乳杆菌乳酸亚种LTI 30的抑制作用更强，但单独使用嗜酸菌素CH5时抑制水平最高。向MRS肉汤中添加嗜酸菌素CH5和超过30 mg/ml的溶菌酶，抑制水平高于单独使用嗜酸菌素CH5时的水平。指示菌德氏乳杆菌乳酸亚种LTI 30在MRS肉汤和RSM中对NaCl、NaNO₃和溶菌酶也敏感。
