Diez-Gonzalez F, Russell J B, Hunter J B
Agricultural Research Service, USDA and Section of Microbiology, Wing Hall, Cornell University, Ithaca, NY 14853, USA.
Arch Microbiol. 1996 Dec;166(6):418-20. doi: 10.1007/BF01682990.
Clostridum acetobutylicum strain P262 fermented glucose, pyruvate, or lactate, and the butyrate production was substrate-dependent. Differences in butyrate yield could not be explained by changes in butyrate kinase activities, but the butyrate production was inversely related to acetate kinase activity. The acetate kinase had a pH optimum of 8.0, a Km for acetate of 160 mM, and a kcat of 16, 800 min-1. The enyzme had a native molecular mass of 78 kDa; the size of 42 kDa on SDS-PAGE indicated that the acetate kinase of strain P262 was a homodimer.
丙酮丁醇梭菌P262菌株可发酵葡萄糖、丙酮酸或乳酸，丁酸盐的产生取决于底物。丁酸盐产量的差异无法用丁酸盐激酶活性的变化来解释，但丁酸盐的产生与乙酸盐激酶活性呈负相关。乙酸盐激酶的最适pH为8.0，乙酸盐的Km为160 mM，kcat为16800 min-1。该酶的天然分子量为78 kDa；SDS-PAGE上42 kDa的大小表明P262菌株的乙酸盐激酶是一种同型二聚体。
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