Rosner B M, Rainey F A, Kroppenstedt R M, Schink B
Fakultät für Biologie, Universität Konstanz, Germany.
FEMS Microbiol Lett. 1997 Mar 15;148(2):175-80. doi: 10.1111/j.1574-6968.1997.tb10285.x.
Aerobic acetylene-degrading bacteria were isolated from soil samples. Two isolates were assigned to the species Rhodococcus opacus, two others to Rhodococcus ruber and Gordona sp. They were compared with known strains of aerobic acetylene-, cyanide-, or nitrile-utilizing bacteria. The acetylene hydratases of R opacus could be measured in cell-free extracts only in the presence of a strong reductant like titanium(III) citrate. Expression of these enzymes was molybdenum-dependent. Acetylene hydratases in cell-free extracts of R ruber and Gordona spp. did not require addition of reductants. No cross-reactivity could be found between cell-free extracts of any of these aerobic isolates and antibodies raised against the acetylene hydratase of the strictly anaerobic fermenting bacterium Pelobacter acetylenicus. These results show that acetylene hydratases are a biochemically heterogeneous group of enzymes.
从土壤样本中分离出了好氧乙炔降解细菌。其中两株被鉴定为不透明红球菌，另外两株为红球菌和戈登氏菌属。将它们与已知的利用乙炔、氰化物或腈的好氧细菌菌株进行了比较。仅在存在强还原剂如柠檬酸钛（III）的情况下，才能在无细胞提取物中测量不透明红球菌的乙炔水合酶。这些酶的表达依赖于钼。红球菌和戈登氏菌属无细胞提取物中的乙炔水合酶不需要添加还原剂。在这些好氧分离株的任何无细胞提取物与针对严格厌氧发酵细菌乙炔杆菌的乙炔水合酶产生的抗体之间未发现交叉反应。这些结果表明，乙炔水合酶是一组生化性质不同的酶。
