sp. nov., a diazotrophic, acetylenotrophic anaerobe isolated from intertidal sediments.

A Gram-stain-negative, strictly anaerobic, non-motile, rod-shaped bacterium, designated SFB93, was isolated from the intertidal sediments of South San Francisco Bay, located near Palo Alto, CA, USA. SFB93 was capable of acetylenotrophic and diazotrophic growth, grew at 22-37 °C, pH 6.3-8.5 and in the presence of 10-45 g l NaCl. Phylogenetic analyses based on 16S rRNA gene sequencing showed that SFB93 represented a member of the genus with highest 16S rRNA gene sequence similarities to DSM 3246 (96.6 %), DSM 2380 (96.5 %), and DSM 2394 (96.7 %). Genome sequencing revealed a genome size of 3.22 Mbp and a DNA G+C content of 53.4 %. SFB93 had low genome-wide average nucleotide identity (81-87.5 %) and <70 % digital DNA-DNA hybridization value with other members of the genus . The phylogenetic position of SFB93 within the family and as a novel member of the genus was confirmed via phylogenetic reconstruction based on concatenated alignments of 92 bacterial core genes. On the basis of the results of phenotypic, genotypic and phylogenetic analyses, a novel species, sp. nov., is proposed, with SFB93 (=DSM 106009=JCM 33327=ATCC TSD-118) as the type strain.