Pacheco A B, Guth B E, Soares K C, de Almeida D F, Ferreira L C
Laboratório de Fisiologia Celular, Universidade Federal do Rio de Janeiro, CCS, Cidade Universitária, Brazil.
FEMS Microbiol Lett. 1997 Mar 15;148(2):255-60. doi: 10.1111/j.1574-6968.1997.tb10297.x.
The genetic diversity in a group of Escherichia coli strains belonging to serogroup O6 but expressing different H antigens was investigated by random amplification of polymorphic DNA (RAPD). Isolates of serotypes H16, H1, H31, and non-motile (NM) strains were typed using a set of 3 primers with different G + C contents. The amplified band arrays allowed the identification of 3 main clonal clusters corresponding to each O:H serotype analyzed. Based on their RAPD profiles NM strains could be assigned to either H1 or H31 serotypes. The results indicate that the flagellar antigen and the RAPD fingerprint represent reliable clonal markers in this E. coli group.
通过随机扩增多态性DNA（RAPD）技术，对一组属于O6血清群但表达不同H抗原的大肠杆菌菌株的遗传多样性进行了研究。使用一组具有不同G + C含量的3种引物对H16、H1、H31血清型的分离株以及无动力（NM）菌株进行分型。扩增带型阵列能够鉴定出与所分析的每种O:H血清型相对应的3个主要克隆簇。根据其RAPD图谱，NM菌株可归为H1或H31血清型。结果表明，鞭毛抗原和RAPD指纹图谱是该组大肠杆菌中可靠的克隆标记。
