通过随机扩增多态性DNA分析揭示产肠毒素大肠杆菌O6:H16血清型的克隆性质
Clonal nature of enterotoxigenic Escherichia coli serotype O6:H16 revealed by randomly amplified polymorphic DNA analysis.
作者信息
Pacheco A B, Soares K C, de Almeida D F, Viboud G I, Binsztein N, Ferreira L C
机构信息
Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS, Cidade Universitária, Brazil.
出版信息
J Clin Microbiol. 1998 Jul;36(7):2099-102. doi: 10.1128/JCM.36.7.2099-2102.1998.
Abstract
The genetic relatedness among 29 enterotoxigenic Escherichia coli strains of serotype O6:H16 was investigated by randomly amplified polymorphic DNA (RAPD) analysis. The strains were isolated in different parts of the world, displayed CS1-CS3 or CS2-CS3 profiles, and expressed heat-labile toxin (LT) and heat-stable toxin; a single strain expressed only LT. Ten RAPD types were distinguished and showed significant similarity, having on average 82% of the amplified bands in common. These results indicated that, irrespective of the different geographical origin or virulence factors, these strains belonged to a widespread clonal group.
摘要
通过随机扩增多态性DNA（RAPD）分析，研究了29株O6:H16血清型产肠毒素大肠杆菌菌株之间的遗传相关性。这些菌株分离于世界不同地区，呈现CS1-CS3或CS2-CS3图谱，并表达不耐热毒素（LT）和耐热毒素；仅有一株菌株仅表达LT。区分出了10种RAPD类型，它们显示出显著的相似性，平均共有82%的扩增条带。这些结果表明，无论地理来源或毒力因子如何不同，这些菌株都属于一个广泛分布的克隆群。
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本文引用的文献
1
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Clin Microbiol Rev. 1997 Oct;10(4):569-84. doi: 10.1128/CMR.10.4.569.
2
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J Clin Microbiol. 1997 Jun;35(6):1521-5. doi: 10.1128/jcm.35.6.1521-1525.1997.
3
Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD.基于随机扩增多态性DNA分析的大肠杆菌O6血清型分离株间的克隆关系
FEMS Microbiol Lett. 1997 Mar 15;148(2):255-60. doi: 10.1111/j.1574-6968.1997.tb10297.x.
4
Colonization factors of enterotoxigenic Escherichia coli isolated from children in north India.从印度北部儿童中分离出的产肠毒素大肠杆菌的定植因子。
J Infect Dis. 1996 Oct;174(4):768-76. doi: 10.1093/infdis/174.4.768.
5
Characterization of enterotoxigenic Escherichia coli isolated from U.S. troops deployed to the Middle East.从部署到中东的美军中分离出的产肠毒素大肠杆菌的特性分析。
J Clin Microbiol. 1993 Apr;31(4):851-6. doi: 10.1128/jcm.31.4.851-856.1993.
6
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Nucleic Acids Res. 1993 Dec 25;21(25):5930-3. doi: 10.1093/nar/21.25.5930.
7
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J Clin Microbiol. 1993 Mar;31(3):558-64. doi: 10.1128/jcm.31.3.558-564.1993.
8
Serotypes of enterotoxigenic Escherichia coli in Thailand and the Philippines.泰国和菲律宾产肠毒素大肠杆菌的血清型
Infect Immun. 1982 Jun;36(3):851-6. doi: 10.1128/iai.36.3.851-856.1982.
9
Hemagglutination activity and colonization factor antigens I and II in enterotoxigenic and non-enterotoxigenic strains of Escherichia coli isolated from humans.从人类分离出的产肠毒素和非产肠毒素大肠杆菌菌株中的血凝活性以及定居因子抗原I和II
Infect Immun. 1982 Apr;36(1):189-97. doi: 10.1128/iai.36.1.189-197.1982.
10
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