Fu E, Nieh S, Chang H L, Wang S L
Department of Periodontology, National Defense Medical Center, School of Dentistry, Taipei, Taiwan, Republic of China.
Int J Periodontics Restorative Dent. 1996 Jun;16(3):278-91.
Because the existence of cyclosporin A-induced gingival overgrowth in animals has not been well established, a pilot study was undertaken to determine whether such gingival overgrowth could be observed in Sprague-Dawley rats. Thirty-six male rats, age 6 weeks, were randomly divided into two groups. The test group was given 30 mg/kg of body weight of cyclosporin A daily by gastric feeding. The control group was given mineral oil instead. Stone casts of the gingiva in the mandibular incisor region were made biweekly for 6 weeks. The three-dimensional growth pattern-the buccolingual width, the mesiodistal width, and the vertical height of the gingiva-was analyzed on the casts. The animals were killed after the last impression was taken, and tissue sections were made. Stereomicroscopy revealed marked overgrowth of the gingiva in the test rats. All three-dimensional measurements on the stone casts were greater in the test group, beginning 2 weeks after cyclosporin A was given. Histologically the overgrowth of the epithelia and connective tissue was easily confirmed in the buccal and lingual gingiva of the test rats.
由于环孢素A诱导的动物牙龈过度生长的存在尚未得到充分证实，因此进行了一项初步研究，以确定在斯普拉格-道利大鼠中是否可以观察到这种牙龈过度生长。36只6周龄的雄性大鼠被随机分为两组。试验组通过胃饲法每天给予30mg/kg体重的环孢素A。对照组则给予矿物油。在6周内每两周制作一次下颌切牙区域牙龈的石膏模型。在模型上分析牙龈的三维生长模式——颊舌宽度、近远中宽度和垂直高度。在最后一次取模后处死动物，并制作组织切片。立体显微镜检查显示试验大鼠牙龈明显过度生长。从给予环孢素A后2周开始，试验组在石膏模型上的所有三维测量值都更大。组织学上，在试验大鼠的颊侧和舌侧牙龈中很容易证实上皮和结缔组织的过度生长。
