Diaz R S, Zhang L, Busch M P, Mosley J W, Mayer A
Irwin Memorial Blood Centers, San Francisco, CA 94118, USA.
AIDS. 1997 Mar 15;11(4):415-22. doi: 10.1097/00002030-199704000-00003.
During treatment with blood components prepared from an HIV-infected donation, two recipients became infected in 1985. One recipient infected her sexual partner.
To evaluate the evolution of the originally-shared HIV-1 quasispecies in different human hosts over time, sequence data were obtained from serum from the actual donation sample of blood, and from plasma samples collected from the four members of the epidemiologic cluster over a period extending from 1986 to 1993.
The V3 hypervariable region of env and the gag p17 gene were analysed. CD4 and CD8 counts, as well as HIV RNA burden data, were collected.
One patient died from AIDS during the study. This patient showed a greater degree of diversity in the V3 region, with a higher positive charge over time, than the other individuals. Phylogenetic analysis revealed that the V3 sequences from each of the four individuals occupied separate branches of a phylogenetic reconstruction (tree). Two distinct subgroups evolved in the donor, one with GPGR and the other with GSGR/GSGK at the tip of the V3 loop. This latter group was not detected in the other individuals. The sequences in the sexual partner were no more related to those in the infecting transfusion recipient than to sequences from the other members of the cluster, consistent with sexual transmission having occurred at a time shortly after the recipient was infected.
The shared HIV-1 quasispecies in this epidemiologic cluster diverged in an individual-specific manner.
1985年，在使用从一名感染HIV的献血者所制备的血液成分进行治疗期间，两名接受者被感染。一名接受者将病毒传染给了她的性伴侣。
为了评估最初共享的HIV-1准种在不同人类宿主中随时间的演变情况，从实际献血样本的血清以及在1986年至1993年期间从该流行病学群组的四名成员采集的血浆样本中获取了序列数据。
分析了env基因的V3高变区和gag p17基因。收集了CD4和CD8细胞计数以及HIV RNA载量数据。
在研究期间，一名患者死于艾滋病。与其他个体相比，该患者的V3区域显示出更大程度的多样性，且随着时间推移带正电荷更高。系统发育分析表明，来自这四个人的V3序列在系统发育重建（树）中占据不同的分支。在献血者中形成了两个不同的亚组，一个在V3环顶端具有GPGR，另一个具有GSGR/GSGK。在其他个体中未检测到后一组。性伴侣的序列与感染性输血接受者的序列之间的相关性并不高于与该群组其他成员序列的相关性，这与在接受者被感染后不久发生性传播一致。
该流行病学群组中共享的HIV-1准种以个体特异性方式发生了分化。
