Oh-hashi K, Watanabe S, Kobayashi T, Okuyama H
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Biol Pharm Bull. 1997 Mar;20(3):217-23. doi: 10.1248/bpb.20.217.
Previously, we reported that a high alpha-linolenate [18:3(n-3)] diet compared with a high linoleate [18:2(n-6)] diet suppressed the anti-egg albumin (EA) immunoglobulin E (IgE) antibody response in mice. Because of relatively high background values obtained with the method used previously, we used an improved ELISA and once again determined serum IgE levels. In contrast to our previous results, the serum level of anti-dinitrophenyl specific (anti-DNP) as well as total IgE in mice immunized with DNP-antigen was slightly but significantly higher in the high alpha-linolenate diet group than in the high linoleate diet group. Anti-DNP IgG1 and IgG2a antibody responses were not significantly different in mice fed these diets. Indomethacin administration during immunization tended to enhance the IgE antibody responses. The mortality of mice from antigen-induced anaphylactic shock was significantly lower in the high alpha-linolenate diet group than in the high linoleate diet group; however, there was no difference between the groups in terms of vascular permeability and histamine levels. Thus, the high alpha-linolenate diet enhances the IgE antibody response slightly without affecting either the IgG antibody response, vascular permeability or histamine release. The high alpha-linolenate diet possibly suppresses anaphylactic shock by reducing the synthesis of lipid mediators such as eicosanoids and platelet-activating factor.
此前，我们报道过，与高亚油酸[18:2(n-6)]饮食相比，高α-亚麻酸[18:3(n-3)]饮食可抑制小鼠抗卵清蛋白（EA）免疫球蛋白E（IgE）抗体反应。由于之前使用的方法获得的背景值相对较高，我们采用了改进的酶联免疫吸附测定（ELISA）法，并再次测定血清IgE水平。与我们之前的结果相反，在高α-亚麻酸饮食组中，用二硝基苯基特异性抗原（抗-DNP）免疫的小鼠血清中抗-DNP水平以及总IgE水平略高但显著高于高亚油酸饮食组。喂食这些饮食的小鼠中，抗-DNP IgG1和IgG2a抗体反应没有显著差异。免疫期间给予吲哚美辛倾向于增强IgE抗体反应。高α-亚麻酸饮食组中因抗原诱导的过敏性休克导致的小鼠死亡率显著低于高亚油酸饮食组；然而，两组在血管通透性和组胺水平方面没有差异。因此，高α-亚麻酸饮食在不影响IgG抗体反应、血管通透性或组胺释放的情况下，略微增强了IgE抗体反应。高α-亚麻酸饮食可能通过减少类花生酸和血小板活化因子等脂质介质的合成来抑制过敏性休克。
