Lee J E, Choi S Y, Suk J W, Hong J W, Yoo B K, Choi E Y, Jang S H, Park K A, Cho S W
Department of Anatomy, Yonsei University College of Medicine, Seoul, Korea.
Mol Cells. 1997 Feb 28;7(1):13-20.
Succinic semialdehyde reductase (SSR) that catalyzes the reduction of succinic semialdehyde (SSA) to gamma-hydroxybutyrate (GHB) has been identified as one of the NADPH-dependent aldehyde reductases. Reduction of SSA to GHB strongly supports the proposal that GHB biosynthesis may be an important step in the GABA shunt. It is pharmacologically significant in anesthesia, evoking the state of sleep, and an increase in brain dopamine level. Monoclonal antibodies against bovine brain succinic semialdehyde reductase were produced. Using the anti-succinic semialdehyde reductase antibodies, we investigated the distribution of brain succinic semialdehyde reductase in rat brain. The brain tissues were sectioned with a basis on the rat brain atlas of Paxinos and were stained by the immunoperoxidase staining method using monoclonal antibodies. In the section of the frontal lobe, immunoreactive cells were observed in the lateral septal area, the ventral pallidum, which belongs to the substantia innominata. We could observe immunoreactive cells in the reticular thalamic nucleus, which is closely related with 'sleeping', the basal nuclei of Meynert, which is associated with Alzheimer's disease, and hypothalamic nuclei. Immunoreactive cells were also shown in raphe nuclei or the reticular formation of the midbrain, cerebellum, and inferior olivary nuclei of the medulla oblongata. Succinic semialdehyde reductase-immunoreactive cells were distributed extensively in rat brain, especially immunoreactive cells were strongly observed in the areas associated with the limbic system and reticular formation.
催化琥珀酸半醛（SSA）还原为γ-羟基丁酸（GHB）的琥珀酸半醛还原酶（SSR）已被确定为NADPH依赖性醛还原酶之一。SSA还原为GHB有力地支持了GHB生物合成可能是γ-氨基丁酸（GABA）分流重要步骤的观点。它在麻醉、诱发睡眠状态以及提高脑多巴胺水平方面具有药理学意义。制备了针对牛脑琥珀酸半醛还原酶的单克隆抗体。利用抗琥珀酸半醛还原酶抗体，我们研究了大鼠脑中琥珀酸半醛还原酶的分布。根据帕西诺斯大鼠脑图谱对脑组织进行切片，并用单克隆抗体通过免疫过氧化物酶染色法进行染色。在额叶切片中，在外侧隔区、属于无名质的腹侧苍白球观察到免疫反应性细胞。我们可以在与“睡眠”密切相关的丘脑网状核、与阿尔茨海默病相关的迈内特基底核以及下丘脑核中观察到免疫反应性细胞。在中脑的中缝核或网状结构、小脑以及延髓的下橄榄核中也显示出免疫反应性细胞。琥珀酸半醛还原酶免疫反应性细胞广泛分布于大鼠脑中，尤其是在与边缘系统和网状结构相关的区域中强烈观察到免疫反应性细胞。
