Simon M R, Silva J, Freier D, Bruner J, Wiliams R
Infect Immun. 1977 Oct;18(1):73-7. doi: 10.1128/iai.18.1.73-77.1977.
Five mongrel dogs were sensitized to tuberculoprotein with Freund adjuvant, as determined by responses to tuberculin skin tests and by in vitro lymphocyte cultures. These animals served as blood donors for production of transfer factor. Nine mongrel dogs received single doses of transfer factor that was tuberculin specific. Successful transfer of tuberculin sensitivity as measured by lymphocyte transformations was achieved in five of nine animals. Canine lymphocyte cultures frequently showed relatively low transformations to mitogens as compared with responses noted in other animals. Dissociation between skin test reactivity and in vitro lymphocyte transformation to tuberculoprotein was noted in two of nine animals. Increases in lymphocyte transformations to phytochemagglutinin were seen in three of nine recipient animals after transfer factor administration. Successful transfer from an animal sensitized by prior transfer factor administration was achieved in one instance. These data indicate that transfer factor-like substances are present in dogs. The reasons why successful transfer was achieved in only half of the recipient animals needs further explanation.
通过结核菌素皮肤试验反应和体外淋巴细胞培养确定，五只杂种犬用弗氏佐剂对结核蛋白致敏。这些动物作为生产转移因子的供血者。九只杂种犬接受了单剂量的结核菌素特异性转移因子。通过淋巴细胞转化测定，九只动物中有五只成功实现了结核菌素敏感性的转移。与其他动物的反应相比，犬淋巴细胞培养对有丝分裂原的转化通常较低。九只动物中有两只出现皮肤试验反应性与体外淋巴细胞对结核蛋白转化之间的分离。九只接受转移因子的动物中有三只在给予转移因子后淋巴细胞对植物血凝素的转化增加。在一个实例中，成功地从先前给予转移因子致敏的动物进行了转移。这些数据表明犬体内存在类似转移因子的物质。仅一半接受动物成功实现转移的原因需要进一步解释。
