Giambrone J J, Klesius P H, Yu M
Poult Sci. 1983 May;62(5):767-71. doi: 10.3382/ps.0620767.
Delayed wattle reactions (DWR) to tuberculin, diphtheria toxoid (DT), and keyhole limpet hemocyanin (KLH) were transferred to chickens with dialyzable leukocyte extracts (DLE) prepared from splenic leukocytes of chickens sensitized and reactive by DWR to the tuberculin, DT, or KLH. A DLE prepared from chickens unsensitized and unreactive to tuberculin by DWR failed to transfer DWR to tuberculin in recipients. Only chickens injected with DLE from tuberculin sensitized and reactive chickens exhibited significant (P less than .01) DWR to tuberculin. Chickens that received DLE prepared from DT sensitized and reactive chickens exhibited significant (P less than .01) DWR to DT but not to KLH. Further, DWR to both DT and KLH was transferred with DLE prepared from chickens sensitized to both antigens. Adoptive transfer of DWR to KLH in comparison to DT was more successful. Finally, the serial transfer of DWR to KLH (P less than .05) but not to DT (P greater than .05) was accomplished using DLE prepared from chickens that previously were recipients of DLE prepared from chickens sensitized to KLH and DT. Results indicate that DLE prepared from chickens contain transfer factor (TF) responsible for adoptive transfer of DWR to tuberculin, DT, and KLH.
对结核菌素、白喉类毒素（DT）和钥孔戚血蓝蛋白（KLH）的延迟肉垂反应（DWR），通过用可透析白细胞提取物（DLE）从经DWR致敏并对结核菌素、DT或KLH有反应的鸡的脾脏白细胞制备而成，转移到鸡身上。由对结核菌素无致敏反应且无DWR反应的鸡制备的DLE未能将对结核菌素的DWR转移到受体鸡身上。只有注射了来自结核菌素致敏且有反应的鸡的DLE的鸡，才表现出对结核菌素的显著（P小于0.01）DWR。接受来自DT致敏且有反应的鸡制备的DLE的鸡，表现出对DT的显著（P小于0.01）DWR，但对KLH无显著反应。此外，对DT和KLH的DWR都可通过用对两种抗原都致敏的鸡制备的DLE进行转移。与DT相比，DWR对KLH的过继转移更成功。最后，使用从先前接受过来自对KLH和DT致敏的鸡制备的DLE的鸡制备的DLE，完成了对KLH的DWR的连续转移（P小于0.05），但对DT的转移未成功（P大于0.05）。结果表明，从鸡制备的DLE含有负责将DWR过继转移到结核菌素、DT和KLH的转移因子（TF）。
