Shoji N, Oshika T, Masuda K
Department of Ophthalmology, Musashino Red Cross Hospital, Tokyo, Japan.
Nippon Ganka Gakkai Zasshi. 1997 Mar;101(3):209-14.
We measured the time course of aqueous protein concentration (APC) with a laser flare-cell meter after the injection of endothelin-1 (ET-1) into the vitreous cavity of pigmented rabbits and investigated the influence of pre- or post-treatment with an anti-prostaglandin agent on these effects of ET-1. Injection of ET-1 significantly increased APC in a dose-dependent fashion. After 10(-5)M ET-1 injection, APC reached maximum at 4 hours after treatment and returned to the normal level 24 hours after the injection. On the other hand, the 10(-4)M ET-1 model displayed a bi-phase time course, with a peak value observed at 4 approximately 8 hours and 48 hours post-treatment, and APC did not return to normal even 7 days after treatment. Treatment with anti-prostaglandin agents before and after the injection blocked APC increase completely in the 10(-5)M ET-1 model, and partially in the 10(-4)M ET-1 model. These results indicate that ET-1 effects on APC are at least partially mediated by the cyclooxygenase pathway of the arachidonic acid cascade.
我们在向有色家兔的玻璃体腔注射内皮素-1（ET-1）后，用激光散射细胞仪测量了房水蛋白浓度（APC）的时间进程，并研究了用抗前列腺素药物进行预处理或后处理对ET-1这些效应的影响。注射ET-1后，APC以剂量依赖方式显著增加。注射10(-5)M ET-1后，APC在处理后4小时达到最大值，并在注射后24小时恢复到正常水平。另一方面，10(-4)M ET-1模型呈现双相时间进程，在处理后约4至8小时和48小时观察到峰值，甚至在处理7天后APC仍未恢复正常。在注射前后用抗前列腺素药物处理，在10(-5)M ET-1模型中完全阻断了APC的增加，在10(-4)M ET-1模型中部分阻断了APC的增加。这些结果表明，ET-1对APC的作用至少部分是由花生四烯酸级联反应的环氧化酶途径介导的。
