Shoji N, Oshika T, Masuda K
Department of Ophthalmology, Musashino Red Cross Hospital, Tokyo, Japan.
Nippon Ganka Gakkai Zasshi. 1995 Jun;99(6):631-5.
We assessed the role of endothelin-1 (ET-1) in the development of anterior chamber inflammation in pigmented rabbit eyes. After the injection of ET-1 (10(-13), 10(-11), 10(-9) or 10(-7) M) into the anterior chamber, aqueous protein concentration increased significantly in a dose-dependent fashion, with a peak at 1 to 2 hours after treatment, and it returned to the normal level 8 hours after the injection. Pupillary diameter was significantly reduced when 10(-9) M or 10(-7) M ET-1 solution was injected. These effects were blocked by pretreatment with anti-prostaglandin agents, i.e., topical indomethacin or venous diclofenac sodium. In the endotoxin-induced experimental uveitis model, ET-1 concentration in the aqueous humor was significantly higher than that of normal controls as well as the plasma ET-1 level. These results suggest that ET-1 is an important mediator in the ocular inflammatory reactions through the arachidonic acid cascade.
我们评估了内皮素 -1（ET -1）在色素沉着兔眼前房炎症发展中的作用。向前房注射ET -1（10^(-13)、10^(-11)、10^(-9)或10^(-7) M）后，房水蛋白浓度呈剂量依赖性显著增加，治疗后1至2小时达到峰值，注射后8小时恢复至正常水平。注射10^(-9) M或10^(-7) M ET -1溶液时，瞳孔直径显著减小。这些效应可被前列腺素拮抗剂预处理阻断，即局部应用吲哚美辛或静脉注射双氯芬酸钠。在内毒素诱导的实验性葡萄膜炎模型中，房水中ET -1浓度显著高于正常对照组以及血浆ET -1水平。这些结果表明，ET -1是通过花生四烯酸级联反应参与眼部炎症反应的重要介质。
