Kurazono T, Makino S
Saitama Institute of Public Health, Division of Clinical Microbiology.
Nihon Rinsho. 1997 Mar;55(3):671-4.
A random amplified polymorphic DNA(RAPD) fingerprinting method has been developed to differentiate Enterohemorrhagic Escherichia coli(EHEC) O157:H7 isolates. This method uses an oligonucleotide of arbitrarily chosen sequence to prime DNA synthesis from pairs of sites to which it is matched or practically matched and results in strain-specific arrays of DNA products. By the RAPD analysis using A07(5'-TGCCTCGCACCA-3'), EHEC strains tested in this study were found to be divided into 5 groups. The RAPD arrays among 5EHEC clinical isolates from a single outbreak were identical from each other, and different from other origin. The data indicate that the RAPD method is feasible for investigating the source of an outbreak associated with EHEC O157:H7 infection.
已开发出一种随机扩增多态性DNA（RAPD）指纹图谱方法，用于区分肠出血性大肠杆菌（EHEC）O157:H7分离株。该方法使用任意选择序列的寡核苷酸，从与之匹配或实际匹配的位点对引发DNA合成，从而产生菌株特异性的DNA产物阵列。通过使用A07（5'-TGCCTCGCACCA-3'）进行RAPD分析，本研究中测试的EHEC菌株被分为5组。来自单次暴发的5株EHEC临床分离株之间的RAPD阵列彼此相同，且与其他来源的不同。数据表明，RAPD方法对于调查与EHEC O157:H7感染相关的暴发来源是可行的。
