Iyoda S, Wada A, Weller J, Flood S J, Schreiber E, Tucker B, Watanabe H
Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan.
Microbiol Immunol. 1999;43(8):803-6. doi: 10.1111/j.1348-0421.1999.tb02473.x.
The amplified fragment-length polymorphism (AFLPTM) technique is based on the selective PCR amplification of restriction fragments. We investigated the utility of AFLP in the molecular subtyping of enterohemorrhagic Escherichia coli serotype O157:H7 isolates. We analyzed a total of 46 isolates of E. coli O157:H7 along with other serotypes, O26:H11, 0114:H19 and 0119:NT. Isolates of E. coli O157:H7 derived from the same outbreak showed an identical AFLP-banding pattern and were subtyped into the same group, giving results almost consistent with those of a pulsed-field gel electrophoresis (PFGE) study, while other serotypes showed clearly different patterns from those of E. coli O157:H7. These results suggest that the AFLP technique has potential as an alternative tool for the molecular epidemiology of E. coli O157:H7.
扩增片段长度多态性（AFLPTM）技术基于对限制性片段的选择性PCR扩增。我们研究了AFLP在肠出血性大肠杆菌O157:H7血清型分离株分子分型中的应用。我们共分析了46株大肠杆菌O157:H7分离株以及其他血清型，O26:H11、O114:H19和O119:NT。来自同一疫情暴发的大肠杆菌O157:H7分离株显示出相同的AFLP条带模式，并被分为同一组，其结果与脉冲场凝胶电泳（PFGE）研究的结果几乎一致，而其他血清型显示出与大肠杆菌O157:H7明显不同的模式。这些结果表明，AFLP技术有潜力作为大肠杆菌O157:H7分子流行病学的替代工具。
