Clegg J S, Lovallo J
J Cell Physiol. 1977 Oct;93(1):161-7. doi: 10.1002/jcp.1040930120.
The concentration of total ninhydrin-positive material (NPM) soluble in 5% trichloroacetic acid was measured in cysts of the brine shrimp. Artemia salina, as a function of hydration level. No net change in NPM was observed until the cysts had achieved a water content of about 0.65 g H2O/g of initially dry cysts. Above this hydration threshold the NPM content increased markedly. Examination of the free amino acid composition of cysts incubated at selected hydration levels revealed that almost all of the amino acids underwent net change above the hydration threshold. However, just below this threshold, the free amino acid composition was essentially the same as in fully dried cysts. The activity generating net changes in the concentration of free amino acids above the hydration threshold was shown to be metabolic in nature and restricted to the cellular component of the cyst.
测定了卤虫(Artemia salina)休眠卵囊中可溶于5%三氯乙酸的总茚三酮阳性物质(NPM)的浓度,并将其作为水合水平的函数。在休眠卵达到约0.65 g水/g初始干卵囊的含水量之前,未观察到NPM的净变化。高于此水合阈值,NPM含量显著增加。对在选定水合水平下孵育的休眠卵的游离氨基酸组成进行检查发现,几乎所有氨基酸在水合阈值以上都发生了净变化。然而,恰好在该阈值以下,游离氨基酸组成与完全干燥的休眠卵基本相同。结果表明,在水合阈值以上引起游离氨基酸浓度净变化的活性本质上是代谢性的,且仅限于休眠卵的细胞成分。