Gawel N J, Bartlett A C
USDA, ARS, Western Cotton Research Laboratory, Phoenix, Arizona 85040, USA.
Insect Mol Biol. 1993;2(1):33-8. doi: 10.1111/j.1365-2583.1993.tb00123.x.
The B biotype of the sweetpotato whitefly, Bemisia tabaci (Gennadius) has caused over $200 million in crop damage in the US during 1992. The taxonomic classification of the A and B biotypes of B. tabaci is unclear. We used RAPD-PCR to demonstrate DNA differences between the A and B biotypes of this insect. All twenty of the RAPD primers tested distinguished readily between the biotypes. DNA extracted from individual eggs and nymphs showed identical differences. RAPD-based genetic similarity statistics indicate that these two biotypes of B. tabaci were no more similar to each other than to two other whitefly species: bayberry whitefly (Parabemisia myricae) or bandedwinged whitefly (Trialeurodes abutilonea). These results indicate that RAPD-PCR may be useful in distinguishing closely related organisms, but may not be useful in determining higher classification of insects. Before the taxonomic status of B. tabaci biotypes A and B can be determined, results from other genetic, morphological and physiological examinations will have to be compared.
烟粉虱（Bemisia tabaci (Gennadius)）的B生物型在1992年给美国造成了超过2亿美元的作物损失。烟粉虱A生物型和B生物型的分类尚不明确。我们使用随机扩增多态性DNA聚合酶链式反应（RAPD-PCR）来证明这种昆虫A生物型和B生物型之间的DNA差异。所测试的全部20种RAPD引物都能轻易区分这两种生物型。从单个卵和若虫中提取的DNA显示出相同的差异。基于RAPD的遗传相似性统计表明，烟粉虱的这两种生物型彼此之间的相似程度并不高于它们与另外两种粉虱：杨梅粉虱（Parabemisia myricae）或带翅粉虱（Trialeurodes abutilonea）的相似程度。这些结果表明，RAPD-PCR可能有助于区分亲缘关系密切的生物，但可能无助于确定昆虫的高级分类。在确定烟粉虱A生物型和B生物型的分类地位之前，必须比较其他遗传、形态和生理检查的结果。
