Fahmy Inas Farouk, Abou-Ali Rania Mahmoud
Department of Microbial Molecular Biology, Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Giza, Egypt.
Department of Nucleic Acid and Protein Structure, Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Giza, Egypt.
J Genet Eng Biotechnol. 2015 Jun;13(1):87-92. doi: 10.1016/j.jgeb.2014.12.004. Epub 2015 Jan 24.
(Gennadius) (Hemiptera, Aleyrodidae) is considered to be one of the most damaging pests in agriculture, causing severe losses in crops worldwide, affecting the tropical and subtropical regions. Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) was used to assess the genetic diversity between different isolates collected from different regions in Egypt compared with some other worldwide isolates of this insect pest. Out of 12 primers 8 primers from Operon technology have shown to differentiate between 13 collected samples from all over Egypt and some other samples collected from different countries with two other populations representing biotypes A and B collected from the US used for biotype demarcation. Using 13 insect samples, RAPD analysis has produced a total number of 72 markers; about 68 polymorphic markers were revealed. The total number of bands obtained for each primer ranged from 4 to 14 within an average of 9 bands per primer. Of the pair wise combination among fifteen populations Ismailia population showed the highest similarity index (0.947), while US biotype A scored the lowest similarity index (0.326). Two major clusters were formed from the UPGMA dendrogram, which was constructed based on Dice similarity coefficient. RAPD-PCR screening demarcated the whitefly population based on the host species and genetic biotypes. Two major clusters have been revealed as A and B with two other minor clusters A1, A2, and B1, B2. Most of the samples collected from Egypt were clustered together in a minor cluster named A1. A1 group is divided into two sub-groups. A1a comprises the populations from Beni-Sweif in Upper Egypt, Ismailia, Kalyobia, El-Fayoum, Tanta, Kafr El-Sheikh, Alexandria, and A1b comprises Spain and Sudan. Group A1a is clustered together based on their host which belongs to the Cucurbitaceae family while Alexandria was separated individually based on its host which is cauliflower. Through the similarity matrix it could be concluded that the populations of Beni-Sweif, Ismailia, Kalyobia, El-Fayoum, Tanta, Kafr El-Sheikh had 80-90% similarity, while the Banha isolate had 30-40% similarity.
烟粉虱（Gennadius）（半翅目，粉虱科）被认为是农业中最具危害性的害虫之一，在全球范围内给农作物造成严重损失，影响热带和亚热带地区。随机扩增多态性DNA - 聚合酶链反应（RAPD - PCR）被用于评估从埃及不同地区收集的不同分离株之间的遗传多样性，并与该害虫在世界其他地区的一些分离株进行比较。在12种引物中，来自Operon技术公司的8种引物已显示出能够区分从埃及各地收集的13个样本以及从不同国家收集的其他一些样本，另外还有从美国收集的代表生物型A和B的两个种群用于生物型划分。使用13个昆虫样本，RAPD分析共产生了72个标记；揭示了约68个多态性标记。每个引物获得的条带总数在4至14条之间，平均每个引物9条带。在15个种群的两两组合中，伊斯梅利亚种群显示出最高的相似性指数（0.947），而美国生物型A的相似性指数最低（0.326）。基于Dice相似系数构建的UPGMA聚类图形成了两个主要聚类。RAPD - PCR筛选根据寄主物种和遗传生物型对粉虱种群进行了划分。揭示了两个主要聚类A和B以及另外两个次要聚类A1、A2和B1、B2。从埃及收集的大多数样本聚集在一个名为A1的次要聚类中。A1组分为两个亚组。A1a包括上埃及贝尼苏韦夫、伊斯梅利亚、卡卢比亚、法尤姆、坦塔、卡夫尔谢赫、亚历山大的种群，A1b包括西班牙和苏丹的种群。A1a组基于其寄主属于葫芦科而聚集在一起，而亚历山大则因其寄主是花椰菜而单独分开。通过相似性矩阵可以得出结论，贝尼苏韦夫、伊斯梅利亚、卡卢比亚、法尤姆、坦塔、卡夫尔谢赫的种群具有80 - 90%的相似性，而班哈分离株具有30 - 40%的相似性。
