Moya A, Guirao P, Cifuentes D, Beitia F, Cenis J L
Instituto Cavanilles de Biodiversidad y Biología Evolutiva, and Departament de Genètica, Universitat de València, Spain.
Mol Ecol. 2001 Apr;10(4):891-7. doi: 10.1046/j.1365-294x.2001.01221.x.
The genetic structure of six Iberian populations of the whitefly Bemisia tabaci, two of them biotype Q, one biotype B, and the other three a mixture of both, has been studied using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). A total of 336 individuals was completely discriminated by means of 234 scored bands. Separate analyses of molecular variance of haploid males and diploid females using the pairwise number of differences between haplotypes showed that biotypes contribute significantly more to the observed variability than populations within biotypes. On average, gene flow between two biotypes of the same population is lower than between populations of identical biotypes. On the basis of these results and the nondetection under natural conditions of a single hybrid, we consider that both biotypes are genetically isolated under the ecological conditions prevailing in the south Iberian Peninsula. All populations of biotype Q presented similar values of intrapopulational diversity, which were higher than the values shown by populations of biotype B.
利用随机扩增多态性DNA - 聚合酶链反应（RAPD - PCR）研究了伊比利亚半岛六个烟粉虱种群的遗传结构，其中两个为Q生物型，一个为B生物型，另外三个是两者的混合类型。通过234条计分条带，总共336个个体被完全区分开来。使用单倍型之间的成对差异数量对单倍体雄性和二倍体雌性进行分子方差的单独分析表明，生物型对观察到的变异性的贡献显著大于生物型内的种群。平均而言，同一种群的两种生物型之间的基因流低于相同生物型的不同种群之间的基因流。基于这些结果以及在自然条件下未检测到单一杂种的情况，我们认为在伊比利亚半岛南部普遍存在的生态条件下，这两种生物型在遗传上是隔离的。所有Q生物型种群呈现出相似的种群内多样性值，这些值高于B生物型种群所显示的值。
