一种用于多种昆虫物种线粒体DNA PCR扩增的改良引物。
An improved primer for PCR amplification of mitochondrial DNA in a variety of insect species.
作者信息
Roehrdanz R L
机构信息
United States Department of Agriculture, Biosciences Research Laboratory, Fargo, North Dakota 58105, USA.
出版信息
Insect Mol Biol. 1993;2(2):89-91. doi: 10.1111/j.1365-2583.1993.tb00129.x.
Abstract
A PCR primer from the mitochondrial COI gene is described that enhances the amplification of the COI-COII region of insect mtDNA. When used in conjunction with a primer from the COII gene identified by R. Crozier, a 1600-1700 bp segment is amplified in nine species of insects representing the orders Lepidoptera, Diptera, Coleoptera and Hymenoptera.
摘要
描述了一种来自线粒体细胞色素氧化酶亚基I（COI）基因的聚合酶链式反应（PCR）引物，该引物可增强昆虫线粒体DNA（mtDNA）细胞色素氧化酶亚基I-细胞色素氧化酶亚基II（COI-COII）区域的扩增。当与由R. 克罗泽鉴定的来自细胞色素氧化酶亚基II（COII）基因的引物一起使用时，在代表鳞翅目、双翅目、鞘翅目和膜翅目的9种昆虫中扩增出了1600-1700碱基对（bp）的片段。
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