Colonna D M, Meng W, Deal D D, Gowda M, Busija D W
Department of Anesthesia, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27157-1009, USA.
Am J Physiol. 1997 Mar;272(3 Pt 2):H1315-22. doi: 10.1152/ajpheart.1997.272.3.H1315.
Temporary elevations in cortical cerebral blood flow (CBF) accompany cortical spreading depression (CSD) in anesthetized animals. We tested the hypothesis that nitric oxide (NO) is an important promotor of CSD-induced cortical hyperemia in urethan-anesthetized rabbits. CBF was measured at four time points by administration of 15-microm microspheres with the reference withdrawal technique. Intravenous administration of the nonspecific NO synthase (NOS) inhibitor N(omega)-nitro-L-arginine increased mean arterial blood pressure and resting cerebrovascular resistance and attenuated CSD-induced hyperemia. Cortical CBF before intraperitoneal 7-nitroindazole (7-NI), a neuronal NOS inhibitor, was 42 +/- 8 and 124 +/- 19 ml x 100 g(-1) x min(-1) at baseline and during CSD, respectively (P < 0.05 by repeated-measures analysis of variance). After 7-NI administration, mean arterial blood pressure, CBF, and cerebrovascular resistance were unchanged from baseline values; cortical CBF was 38 +/- 4 and 90 +/- 8 ml x 100 g(-1) x min(-1) post-7-NI at rest and during a second CSD, respectively. Similar to N(omega)-nitro-L-arginine, 7-NI decreased the cortical hyperemic response during CSD (P < 0.05 by repeated-measures analysis of variance). We conclude that neuronal NOS promotes the temporary cortical hyperemia observed during CSD.
在麻醉动物中，皮质脑血流量（CBF）的暂时升高伴随着皮质扩散性抑制（CSD）。我们测试了一氧化氮（NO）是乌拉坦麻醉兔中CSD诱导的皮质充血的重要促进因子这一假说。通过使用15微米微球并采用参考抽取技术在四个时间点测量CBF。静脉注射非特异性一氧化氮合酶（NOS）抑制剂N（ω）-硝基-L-精氨酸可增加平均动脉血压和静息脑血管阻力，并减弱CSD诱导的充血。在腹腔注射神经元NOS抑制剂7-硝基吲唑（7-NI）之前，皮质CBF在基线和CSD期间分别为42±8和124±19 ml×100 g-1×min-1（通过重复测量方差分析，P<0.05）。给予7-NI后，平均动脉血压、CBF和脑血管阻力与基线值相比无变化；7-NI给药后，静息和第二次CSD期间皮质CBF分别为38±4和90±8 ml×100 g-1×min-1。与N（ω）-硝基-L-精氨酸相似，7-NI降低了CSD期间的皮质充血反应（通过重复测量方差分析，P<0.05）。我们得出结论，神经元NOS促进了CSD期间观察到的暂时性皮质充血。
