Wilkins B S, Jones D B
University Department of Pathology, Southampton General Hospital, U.K.
Biologicals. 1996 Dec;24(4):313-8. doi: 10.1006/biol.1996.0043.
The stroma of bone marrow is a poorly understood tissue which is believed to have important roles in haemopoietic stem cell maintenance and differentiation. We have undertaken immunohistochemical studies of bone marrow stroma in human long-term bone marrow cultures (hLTBMC) and biopsy specimens in order to characterise the cell and matrix components present. We have found two morphological variants of macrophages to be present consistently in hLTBMC stroma, adherent to a substratum of myofibroblastic cells. Large round macrophages appear to actively phagocytic and are formed in hLTBMC regardless of successful establishment of a myofibroblastic cell layer. Elongated macrophages with dendritic processes appear to be non-phagocytic and form only in the presence of a well-established layer of myofibroblasts. Although the functions of these macrophages are not yet known, they have counterparts within intact human bone marrow and their presence in hLTBMC shows some association with the haemopoietic capacity of the cultures.
骨髓基质是一种了解甚少的组织，据信它在造血干细胞的维持和分化中发挥着重要作用。我们对人长期骨髓培养物（hLTBMC）和活检标本中的骨髓基质进行了免疫组织化学研究，以鉴定其中存在的细胞和基质成分。我们发现，在hLTBMC基质中始终存在两种形态变异的巨噬细胞，它们附着于肌成纤维细胞构成的基质上。大型圆形巨噬细胞似乎具有活跃的吞噬作用，并且在hLTBMC中形成，无论肌成纤维细胞层是否成功建立。具有树突状突起的细长巨噬细胞似乎不具有吞噬作用，并且仅在已建立良好的肌成纤维细胞层存在时形成。尽管这些巨噬细胞的功能尚不清楚，但它们在完整的人骨髓中有对应物，并且它们在hLTBMC中的存在与培养物的造血能力存在一定关联。
