Allen T D, Dexter T M
Scan Electron Microsc. 1983(Pt 4):1851-66.
Long-term liquid cultures of bone marrow support the growth and proliferation of self renewing haemopoietic stem cells and the majority of myeloid lineages. The maturation of granulocyte elements occurs in normal cultures, but this may be shifted to erythropoiesis by the addition of a stimulus such as serum from anaemic mice. The continued production of stem cells and differentiating populations of either granulocytes or erythroid cells is dependent upon the establishment and maintenance of an adherent layer in the cultures which arise from the stromal cells in the original marrow inoculum. To date, the presence of adipocytes, fibroblasts, reticulum cells, and endothelial cells has been established amongst the stromal cells; and neutrophil and basophil granulocytes, megakaryocytes, full erythroid differentiation, and monocytes and macrophages have been identified in the haemopoietic lineages. Interactions at the cellular level appear to occur between the lipid synthesising adipocytes and developing granulocytes, and a central macrophage and erythroblasts, forming an 'in vitro' erythroblastic islet. These associations may form aspects of an 'in vitro' haemopoietically inductive microenvironment.
骨髓的长期液体培养可支持自我更新造血干细胞以及大多数髓系谱系的生长和增殖。粒细胞成分在正常培养中会成熟，但通过添加诸如贫血小鼠血清之类的刺激物，这种成熟可能会转向红细胞生成。干细胞以及粒细胞或红细胞系分化群体的持续产生取决于培养物中由原始骨髓接种物中的基质细胞形成的贴壁层的建立和维持。迄今为止，已确定基质细胞中存在脂肪细胞、成纤维细胞、网状细胞和内皮细胞；并且在造血谱系中已鉴定出中性粒细胞和嗜碱性粒细胞、巨核细胞、完全红细胞分化以及单核细胞和巨噬细胞。在细胞水平上，脂质合成脂肪细胞与发育中的粒细胞之间以及中央巨噬细胞与成红细胞之间似乎会发生相互作用，形成一个“体外”成红细胞岛。这些关联可能构成“体外”造血诱导微环境的某些方面。
