PA/纤溶酶系统参与前基质金属蛋白酶-9的加工以及前基质金属蛋白酶-2激活的第二步过程。
Involvement of PA/plasmin system in the processing of pro-MMP-9 and in the second step of pro-MMP-2 activation.
作者信息
Baramova E N, Bajou K, Remacle A, L'Hoir C, Krell H W, Weidle U H, Noel A, Foidart J M
机构信息
Laboratory of Biology, University of Liege, Belgium.
出版信息
FEBS Lett. 1997 Mar 24;405(2):157-62. doi: 10.1016/s0014-5793(97)00175-0.
Pro-MMP2 activation is a two-step process resulting in (1) an intermediate 64 kDa form generated by the MT1-MMP activity, and (2) a mature 62 kDa form. Addition of plasminogen to HT1080 cells cultured under various conditions, or to their membrane preparation, induced a complete conversion of the intermediate MMP-2 form to the mature one, and processing of pro-MMP-9. The pro-MMP-2 activation was inhibited by plasmin inhibitors and anti-uPA antibody. These results provide evidence for involvement of the PA/plasmin system in the second step of MMP-2 activation.
前基质金属蛋白酶2（Pro-MMP2）的激活是一个两步过程，产生（1）由MT1-MMP活性生成的64 kDa中间形式，以及（2）成熟的62 kDa形式。在各种条件下培养的HT1080细胞或其膜制剂中添加纤溶酶原，可诱导中间MMP-2形式完全转化为成熟形式，并使前基质金属蛋白酶9（pro-MMP-9）发生加工处理。纤溶酶抑制剂和抗尿激酶型纤溶酶原激活物（uPA）抗体可抑制Pro-MMP-2的激活。这些结果为纤溶酶原激活物/纤溶酶系统参与MMP-2激活的第二步提供了证据。
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