Spaulding C C, Walford R L, Effros R B
Department of Pathology and Laboratory medicine, University of California School of Medicine, Los Angeles 90095-1732, USA.
Mech Ageing Dev. 1997 Feb;93(1-3):87-94. doi: 10.1016/s0047-6374(96)01824-6.
TNF-alpha and IL-6 are generally increased in the sera of aged humans and mice. The dysregulation of these cytokines may be critical in autoreactivity and immune dysfunction. In earlier studies we demonstrated that production of TNF-alpha and IL-6 following in vitro stimulation of peritoneal macrophages by LPS was reduced in old compared to young mice, and that dietary caloric restriction (CR) had no effect on the induction of TNF-alpha in this system. In the present study we examined the effects of age and calorie restriction on the constitutive production of both TNF-alpha and IL-6. Serum levels of both cytokines were significantly higher in old versus young mice. However, in old mice subjected to long term CR the serum levels were comparable to those of young mice. The potential involvement of normalization of TNF-alpha and IL-6 levels in the life extension effect of CR are discussed.
肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）通常在老年人类和小鼠的血清中升高。这些细胞因子的失调可能在自身反应性和免疫功能障碍中起关键作用。在早期研究中，我们证明与年轻小鼠相比，老年小鼠经脂多糖（LPS）体外刺激腹膜巨噬细胞后，TNF-α和IL-6的产生减少，并且饮食热量限制（CR）对该系统中TNF-α的诱导没有影响。在本研究中，我们研究了年龄和热量限制对TNF-α和IL-6组成性产生的影响。老年小鼠血清中这两种细胞因子的水平均显著高于年轻小鼠。然而，长期接受CR的老年小鼠血清水平与年轻小鼠相当。讨论了TNF-α和IL-6水平正常化在CR延长寿命作用中的潜在参与情况。
