Bosch M M, Wals-Paap C H, Boon M E
Leiden Cytology and Pathology Laboratory, Leiden, The Netherlands.
Eur J Morphol. 1996;34(2):127-30. doi: 10.1076/ejom.34.2.127.13020.
The two-step vacuum-microwave method simplifies and shortens histoprocessing. Acting margins are small so that fine-tuning is required. Temperature, vacuum and time should be in perfect balance. If not, a "cooking" effect (too high temperature) can easily be detected in the epidermis of skin biopsies. An adverse vacuum effect (when vacuum is obtained too abruptly) produces clefts, first observed where different tissues are contiguous. When time is set too shortly, impregnation will be insufficient, which is best seen in kidney tissue. The lessons from these observations may help future users to be troubleshooters while installing their unit.
两步真空微波法简化并缩短了组织处理过程。操作余量小，因此需要微调。温度、真空度和时间应完美平衡。否则，在皮肤活检的表皮中很容易检测到“烹饪”效应（温度过高）。不利的真空效应（当真空获得过于突然时）会产生裂隙，首先在不同组织相邻处观察到。当时间设置过短时，浸渍将不足，这在肾组织中最为明显。这些观察结果的经验教训可能有助于未来的用户在安装设备时进行故障排除。
