Kok L P, Boon M E
Institute for Theoretical Physics, University of Groningen, The Netherlands.
Histochem J. 1995 May;27(5):411-9.
A novel histoprocessing method for paraffin sections is presented in which the combination of vacuum and microwave exposure is the key element. By exploiting the decrease in boiling temperature under vacuum, the liquid molecules in the tissues have been successfully extracted and exchanged at relatively low temperatures during each of the steps from dehydration, clearing, and impregnation. In this vacuum-microwave method, an extremely short time suffices for the preparation of optimal-quality paraffin blocks. No xylene (but isopropanol instead) was used as the intermediate solvent. Thirty biopsies (thickness 2-4 mm) can be processed in 40 min. In addition, this approach can be used to produce large sections of giant blocks (4 x 6 x 1 cm3) which can be easily cut on a routine microtome due to the optimal paraffin impregnation. These giant blocks do not shrink during this vacuum-microwave histoprocessing.
本文介绍了一种用于石蜡切片的新型组织处理方法，其中真空和微波照射的结合是关键要素。通过利用真空下沸点的降低，在脱水、透明和浸蜡的每个步骤中，组织中的液体分子已在相对较低的温度下成功提取和交换。在这种真空-微波方法中，制备高质量石蜡块所需的时间极短。未使用二甲苯（而是用异丙醇代替）作为中间溶剂。30块活检组织（厚度2 - 4毫米）可在40分钟内处理完毕。此外，这种方法可用于制作大型块状组织（4×6×1立方厘米）的大切片，由于石蜡浸渍效果最佳，这些大切片可以很容易地在常规切片机上切割。在这种真空-微波组织处理过程中，这些大型块状组织不会收缩。
