De Bruyn A, Maras M, Schraml J, Herdewijn P, Contreras R
Laboratory of Organic Chemistry, University of Ghent, Belgium.
FEBS Lett. 1997 Mar 17;405(1):111-3. doi: 10.1016/s0014-5793(97)00146-4.
The primary structure of a novel phosphate-containing oligosaccharide, isolated from T. reesei cellobiohydrolase I, was determined by NMR techniques. The new compound has the same structure as GlcMan7GlcNAc2, but it is extended by one alpha-mannopyranosyl unit (Man-P) through a phosphate link. Three different heteronuclear (31P-1H) NMR techniques were used to prove that the phosphate links the glycosidic site of Man-P with C-6 of unit Man-B. The presence of mannoses linked through a phosphate diester resembles glycosyl synthesis in yeast.
从里氏木霉纤维二糖水解酶I中分离出的一种新型含磷酸寡糖的一级结构，通过核磁共振技术得以确定。该新化合物与GlcMan7GlcNAc2具有相同的结构，但通过一个磷酸酯键连接了一个α-甘露糖吡喃糖基单元（Man-P）而得到延伸。运用三种不同的异核（31P-1H）核磁共振技术来证明磷酸将Man-P的糖苷位点与Man-B单元的C-6相连。通过磷酸二酯连接的甘露糖的存在类似于酵母中的糖基合成。
