NM23蛋白在获得性黑素细胞痣、恶性黑色素瘤及恶性黑色素瘤转移灶中的表达：人体皮肤的免疫组织学评估

Expression of NM23 protein in acquired melanocytic nevi, malignant melanoma and metastases of malignant melanoma: an immunohistological assessment in human skin.

作者信息

Reichrath J, Seyfried P, Braun R, Müller S M, Baum H P, Bahmer F A

机构信息

Hautklinik der Universität des Saarlandes, Homburg/Saar, Germany.

出版信息

Dermatology. 1997;194(2):136-9. doi: 10.1159/000246082.

DOI:10.1159/000246082

Abstract

BACKGROUND

It has been suggested that nm23 may exert metastasis suppressor function in human tumors.

OBJECTIVE

We have analyzed expression of nm23 polypeptide in acquired melanocytic nevi (n = 19), dysplastic nevi (n = 19), malignant melanomas (n = 22) and metastases of malignant melanomas (n = 47) in situ.

METHODS

Nm23 protein was detected immunohistochemically on paraffin sections using the highly sensitive labeled avidin-biotin technique.

RESULTS

We found that (1) nm23 polypeptide is predominantly expressed in the cytoplasmic but also in nuclear and membrane compartments of melanocytic human cells, (2) expression of nm23 protein does not correlate with benign or malignant phenotype in melanocytic tumors of human skin.

CONCLUSION

Our study challenges the hypothesis that nm23 may function in malignant melanomas as a tumor suppressor gene.

摘要

背景

有人提出nm23可能在人类肿瘤中发挥转移抑制功能。

目的

我们分析了nm23多肽在获得性黑素细胞痣（n = 19）、发育异常痣（n = 19）、恶性黑色素瘤（n = 22）及原位恶性黑色素瘤转移灶（n = 47）中的表达情况。

方法

采用高敏感性标记抗生物素蛋白-生物素技术，在石蜡切片上通过免疫组织化学方法检测Nm23蛋白。

结果

我们发现：（1）nm23多肽主要在人类黑素细胞的细胞质中表达，但也在细胞核和细胞膜部分表达；（2）nm23蛋白的表达与人类皮肤黑素细胞肿瘤的良性或恶性表型无关。

结论

我们的研究对nm23可能作为肿瘤抑制基因在恶性黑色素瘤中发挥作用这一假说提出了质疑。