Saitoh K, Takahashi H, Yamamoto M, Kishi H, Parsons P G
Department of Dermatology, Sapporo Medical University School of Medicine, Japan.
Histopathology. 1996 Dec;29(6):497-505. doi: 10.1046/j.1365-2559.1996.d01-537.x.
An inverse correlation between the nm23 RNA level and tumour progression of melanocytes has been reported. To elucidate whether the expression of nm23 gene product in malignant melanoma is also inversely correlated with metastatic potential, conventional prognostic parameters or the tumour suppressor protein p53, immunohisto-chemical studies using a monoclonal antibody against nm23-H1 protein were performed on 138 benign and malignant melanocytic tumours. The expression of nm23 protein was compared with that of p53 protein and conventional clinicopathological prognostic factors. The nm23 protein level in benign melanocytes and metastatic melanoma cells was also studied by Western blot analysis. No significant difference regarding the protein was observed between naevi and melanomas, either at histological or protein levels. The expression correlated with local recurrence within 1 year after surgery, level of invasion and tumour thickness, but no parallels were observed between the nm23 and p53 proteins, suggesting that gene is regulated by independent mechanisms, although located on the same chromosome. There was no inverse correlation between the nm23 protein and melanoma metastasis which suggested that the nm23 protein does not appear to be lost during melanoma metastasis.
据报道，nm23 RNA水平与黑素细胞肿瘤进展呈负相关。为阐明nm23基因产物在恶性黑色素瘤中的表达是否也与转移潜能、传统预后参数或肿瘤抑制蛋白p53呈负相关，我们使用抗nm23-H1蛋白的单克隆抗体对138例良性和恶性黑素细胞肿瘤进行了免疫组织化学研究。将nm23蛋白的表达与p53蛋白的表达及传统临床病理预后因素进行了比较。还通过蛋白质印迹分析研究了良性黑素细胞和转移性黑色素瘤细胞中nm23蛋白的水平。在组织学或蛋白质水平上，痣和黑色素瘤之间在蛋白质方面未观察到显著差异。该表达与术后1年内的局部复发、浸润程度和肿瘤厚度相关，但在nm23和p53蛋白之间未观察到平行关系，这表明该基因虽位于同一条染色体上，但受独立机制调控。nm23蛋白与黑色素瘤转移之间不存在负相关，这表明nm23蛋白在黑色素瘤转移过程中似乎并未丢失。
